反刍动物中性粒细胞对感染IBR的牛肾细胞的抗体依赖性细胞介导的细胞毒性：溶酶体阳离子蛋白的作用。
Antibody-dependent cell-mediated cytotoxicity against IBR-infected bovine kidney cells by ruminant neutrophils: the role of lysosomal cationic protein.
作者信息
Thorne K J, Norman J M, Haydock S F, Lammas D A, Duffus P H
出版信息
Immunology. 1984 Oct;53(2):275-82.
Antibody-dependent cell-mediated cytotoxicity (ADCC) of infectious bovine rhinotracheitis (IBR)-infected bovine kidney cells (MDBK) by neutrophils was demonstrated. Neutrophils from bovine and sheep mammary exudate and peripheral blood, and also from human peripheral blood, were all active in the presence of anti-IBR antibody. The component of the ruminant neutrophil granules which was responsible for cytotoxicity appeared to be cationic protein since purified cationic protein lysed the virus-infected cells and heparin inhibited cytotoxicity. Human neutrophil cytotoxicity to herpes simplex virus (HSV)-infected human Chang liver cells was also inhibited by heparin. Human neutrophil cytotoxicity to IBR-infected bovine kidney cells did not appear to be mediated by cationic protein since it was inhibited by the chelators of oxidative intermediates DMSO, thiourea, tryptophane, benzoate and mannitol, and not by heparin.
已证实中性粒细胞对感染传染性牛鼻气管炎（IBR）的牛肾细胞（MDBK）具有抗体依赖性细胞介导的细胞毒性（ADCC）。来自牛和绵羊乳腺渗出液及外周血以及人外周血的中性粒细胞在抗IBR抗体存在下均具有活性。反刍动物中性粒细胞颗粒中负责细胞毒性的成分似乎是阳离子蛋白，因为纯化的阳离子蛋白可裂解病毒感染的细胞，而肝素可抑制细胞毒性。肝素也可抑制人中性粒细胞对单纯疱疹病毒（HSV）感染的人张氏肝细胞的细胞毒性。人中性粒细胞对IBR感染的牛肾细胞的细胞毒性似乎不是由阳离子蛋白介导的，因为它受到氧化中间体二甲基亚砜、硫脲、色氨酸、苯甲酸盐和甘露醇的螯合剂的抑制，而不是肝素的抑制。
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