Department of Animal Science, IVF Laboratory, Faculty of Agricultural and Engineering Science, Razi University, Kermanshah, Iran.
Department of Animal Science, IVF Laboratory, Faculty of Agricultural and Engineering Science, Razi University, Kermanshah, Iran.
Domest Anim Endocrinol. 2019 Jul;68:25-31. doi: 10.1016/j.domaniend.2018.12.010. Epub 2019 Jan 5.
The present study aimed to examine the effects of adding different concentrations of resveratrol during in vitro culture (IVC) alone and during both in vitro maturation (IVM) and IVC on ovine blastocyst yield and quality. Therefore, this study was conducted in two separate experiments. The first experiment was carried out to test the effect of different concentrations of resveratrol (0, 0.1, 0.25, 0.5, 2.0, and 5.0 μM) in the IVC medium on cleavage, morula, developmental potential of blastocyst, and total cell number (TCN) of the embryos. Addition of 0.25 and 0.5 μM of resveratrol during IVC significantly enhanced morula and blastocyst rates as compared with other groups (P < 0.05). Also, supplementation of the IVC medium with 0.5 μM of resveratrol had beneficial effects on trophectoderm cells (TE), inner cell mass (ICM), and TCN of blastocysts. In the second experiment, the same concentrations of resveratrol (0, 0.1, 0.25, 0.5, 2.0, and 5.0 μM) were applied during IVM and IVC. Therefore, oocytes were matured in vitro in the presence of different concentrations of resveratrol for 22-24 h. After in vitro fertilization, presumptive zygotes were cultured in media containing 0, 0.1, 0.25, 0.5, 2.0, and 5.0 μM of resveratrol for 8 d. No significant difference was found in the percentage of oocytes developed to MII (0, 0.1, 0.25, 0.5, and 2.0 μM of resveratrol), but the percentage of oocytes developed to MII were significantly lower in 5.0 μM of resveratrol in comparison with other groups. Addition of 0.5 μM of resveratrol to the maturation and culture media significantly increased morula and blastocyst rates compared with other groups (P < 0.05). However, a too high concentration of resveratrol (5.0 μM) during IVM and IVC decreased cleavage, morula, and blastocyst rates compared with low concentrations (P < 0.05). Treatment with 0.5/0.5 μM of resveratrol during IVM/IVC significantly improved the TE, ICM, and TCN of blastocysts. In conclusion, sequential treatment with 0.5 μM of resveratrol during IVM and IVC and during IVC alone improved the developmental competence of oocytes, which was reflected in higher blastocyst rates and TCN of blastocysts.
本研究旨在探讨在体外培养（IVC）期间以及在体外成熟（IVM）和 IVC 期间添加不同浓度白藜芦醇对绵羊囊胚产量和质量的影响。因此，本研究分为两个独立的实验进行。
第一个实验是为了测试不同浓度（0、0.1、0.25、0.5、2.0 和 5.0μM）的白藜芦醇在 IVC 培养基中对卵裂、桑椹胚、囊胚发育潜力和胚胎总细胞数（TCN）的影响。与其他组相比，添加 0.25 和 0.5μM 的白藜芦醇在 IVC 期间显著提高了桑椹胚和囊胚的比率（P<0.05）。此外，在 IVC 培养基中补充 0.5μM 的白藜芦醇对囊胚的滋养层细胞（TE）、内细胞团（ICM）和 TCN 有有益的影响。
在第二个实验中，应用了相同浓度（0、0.1、0.25、0.5、2.0 和 5.0μM）的白藜芦醇在 IVM 和 IVC 期间。因此，将卵母细胞在不同浓度的白藜芦醇存在下体外成熟 22-24 小时。体外受精后，将假定的受精卵在含有 0、0.1、0.25、0.5、2.0 和 5.0μM 白藜芦醇的培养基中培养 8 天。在 0、0.1、0.25、0.5 和 2.0μM 的白藜芦醇中，卵母细胞发育到 MII 的百分比没有显著差异，但与其他组相比，5.0μM 的白藜芦醇中卵母细胞发育到 MII 的百分比显著降低。与其他组相比，在成熟和培养培养基中添加 0.5μM 的白藜芦醇显著提高了桑椹胚和囊胚的比率（P<0.05）。然而，IVM 和 IVC 期间过高浓度（5.0μM）的白藜芦醇与低浓度相比，降低了卵裂、桑椹胚和囊胚的比率（P<0.05）。在 IVM/IVC 期间用 0.5/0.5μM 的白藜芦醇处理显著改善了囊胚的 TE、ICM 和 TCN。
总之，在 IVM 和 IVC 期间以及在单独的 IVC 期间连续处理 0.5μM 的白藜芦醇提高了卵母细胞的发育能力，这反映在囊胚率和囊胚 TCN 更高。
