Feng Haibo, Fan Jing, Yang Shiping, Zhao Xuelian, Yi Xiao
Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, Sichuan, 610051, PR China.
Int J Biol Macromol. 2017 Jun;99:511-518. doi: 10.1016/j.ijbiomac.2017.02.085. Epub 2017 Feb 24.
Phosphorylated Radix Cyathulae officinalis Kuan polysaccharides (pRCPS) was prepared according to three-factors, ratio of STMP (%) and STPP (%), reaction time and reaction temperature, and three level L(3) orthogonal design. The antiviral activity of nine pRCPS (pRCPS) was systematically evaluated by three methods pre-adding mode, mixed mode, and post-adding mode. Cellular activity was tested by the CCK-8 assay. The results showed that the optimal modification conditions were the ratio of STMP (%) and STPP (%) 1:4, reaction time 2h and reaction temperature 65°C. Six pRCPS (pRCPS, pRCPS, pRCPS) exhibited significant anti-viral activity in pre-adding mode (P<0.05). Eight pRCPS (pRCPS, pRCPS, pRCPS, pRCPS, and pRCPS showed dramatic anti-viral activity in the mixed mode (P<0.05). Six pRCPS (pRCPS, pRCPS, pRCPS) showed antiviral activity in the post-adding mode (P<0.05). Taken together, four pRCPS (pRCPS) demonstrated significant antiviral activity in all the test modes (P<0.05) and their antiviral efficacy were significantly stronger than unmodified RCPS (P<0.05). Those results indicated that four pRCPS (pRCPS) possessed significant antiviral activity and may have potential as a new CPV therapeutic compound, and phosphorylation could significantly enhance the antiviral effect of RCPS. Moreover, phosphorylation modification technique could be valuable as a method to promote the antiviral activity of polysaccharide.
磷酸化川牛膝多糖（pRCPS）是根据三因素（STMP（%）与STPP（%）的比例、反应时间和反应温度）及三水平L(3)正交设计制备的。通过预加样模式、混合模式和后加样模式三种方法系统评价了9种pRCPS的抗病毒活性。采用CCK-8法检测细胞活性。结果表明，最佳修饰条件为STMP（%）与STPP（%）的比例为1:4、反应时间为2小时、反应温度为65℃。6种pRCPS（pRCPS、pRCPS、pRCPS）在预加样模式下表现出显著的抗病毒活性（P<0.05）。8种pRCPS（pRCPS、pRCPS、pRCPS、pRCPS和pRCPS）在混合模式下表现出显著的抗病毒活性（P<0.05）。6种pRCPS（pRCPS、pRCPS、pRCPS）在后加样模式下表现出抗病毒活性（P<0.05）。综合来看，4种pRCPS（pRCPS）在所有测试模式下均表现出显著的抗病毒活性（P<0.05），且其抗病毒效果明显强于未修饰的RCPS（P<0.05）。这些结果表明，四种pRCPS（pRCPS）具有显著的抗病毒活性，可能具有作为新型犬细小病毒治疗化合物的潜力，磷酸化可显著增强RCPS的抗病毒作用。此外，磷酸化修饰技术作为一种促进多糖抗病毒活性的方法可能具有重要价值。
