Feng Haibo, Du Xiaogang, Liu Juan, Han Xingfa, Cao Xiaohan, Zeng Xianyin
Isotope Research Laboratory, College of Life Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China; Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, PR China.
Isotope Research Laboratory, College of Life Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China.
Int J Biol Macromol. 2014 Apr;65:121-8. doi: 10.1016/j.ijbiomac.2014.01.017. Epub 2014 Jan 10.
This study was designed to investigate the effects of oral administration of the polysaccharide from the Radix Cyathulae officinalis Kuan (RCPS) for its adjuvant potential on the specific cellular and humoral immune responses in mice. In this study, our data demonstrated that oral administration of RCPS significantly enhanced the phagocytic capacity of peritoneal macrophage, splenocyte proliferation, the activity of natural killer (NK) cells and cytotoxic T lymphocytes (CTL) and OVA-specific IgG, IgG1, IgG2a, and IgG2b antibody titers. Furthermore, RCPS promoted the level of interleukin-2(IL-2), IFN-γ and IL-4 in CD4(+)T cells and level of IFN-γ in CD8(+)T cells. In addition, RCPS enhanced the expression of CD40(+), CD80(+) and CD86(+) on the dendritic cells (DCs). Importantly, RCPS down-regulated the frequency of CD4(+)CD25(+)Foxp3(+)Treg cells. Taken together, these results suggested that RCPS could increase both cellular and humoral immune responses via up-regulating DCs maturation, and suppressing Treg frequency.
本研究旨在探讨口服川牛膝多糖（RCPS）对小鼠特异性细胞免疫和体液免疫反应的佐剂潜力。在本研究中，我们的数据表明，口服RCPS可显著增强腹腔巨噬细胞的吞噬能力、脾细胞增殖、自然杀伤（NK）细胞和细胞毒性T淋巴细胞（CTL）的活性以及卵清蛋白特异性IgG、IgG1、IgG2a和IgG2b抗体滴度。此外，RCPS可提高CD4(+)T细胞中白细胞介素-2（IL-2）、干扰素-γ（IFN-γ）和IL-4的水平以及CD8(+)T细胞中IFN-γ的水平。此外，RCPS可增强树突状细胞（DC）上CD40(+)、CD80(+)和CD86(+)的表达。重要的是，RCPS可下调CD4(+)CD25(+)Foxp3(+)调节性T细胞（Treg）的频率。综上所述，这些结果表明，RCPS可通过上调DC成熟和抑制Treg频率来增强细胞免疫和体液免疫反应。
