College of life science and technology, Southwest Minzu University, Chengdu, Sichuan 6100041, China.
Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, Sichuan, 610051, China.
Molecules. 2019 Nov 15;24(22):4150. doi: 10.3390/molecules24224150.
This research aimed to investigate the immunomodulatory effects of phosphorylated polysaccharides (pRCPS) in immunosuppressed mice, improving their cellular and humoral immune function. Our results showed that pRCPS increased serum immunoglobulin (IgG, IgA, IgM) concentrations significantly, enhanced splenocyte proliferation, and the thymus and spleen indices. pRCPS also promoted phagocytosis in peritoneal macrophages and enhanced cytokine (IFN-γ, IL-2, -4, -5, -6, and -10) serum levels. Importantly, pRCPS increased the proportions of selected T cell subpopulations (CD3, CD4, and the CD4 to CD8 ratio). Our results revealed that phosphorylation of the polysaccharides promoted their immune-enhancing effects. Thus, pRCPS can enhance cellular and humoral immunity and could be used as an immune-enhancing agent to overcome cyclophosphamide (CY)-induced immunosuppression.
本研究旨在探讨磷酸化多糖（pRCPS）对免疫抑制小鼠的免疫调节作用，提高其细胞和体液免疫功能。我们的结果表明，pRCPS 显著增加了血清免疫球蛋白（IgG、IgA、IgM）浓度，增强了脾细胞增殖以及胸腺和脾脏指数。pRCPS 还促进了腹腔巨噬细胞的吞噬作用，并增强了细胞因子（IFN-γ、IL-2、-4、-5、-6 和-10）的血清水平。重要的是，pRCPS 增加了选定的 T 细胞亚群（CD3、CD4 和 CD4/CD8 比值）的比例。我们的结果表明，多糖的磷酸化促进了其免疫增强作用。因此，pRCPS 可以增强细胞和体液免疫，可作为免疫增强剂来克服环磷酰胺（CY）诱导的免疫抑制。
