测量……中的耗氧率
Measuring Oxygen Consumption Rate in .
作者信息
Palikaras Konstantinos, Tavernarakis Nektarios
机构信息
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece.
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece; Department of Basic Sciences, Faculty of Medicine, University of Crete, Crete, Greece.
出版信息
Bio Protoc. 2016 Dec 5;6(23). doi: 10.21769/BioProtoc.2049.
Abstract
The rate of oxygen consumption is a vital marker indicating cellular function during lifetime under normal or metabolically challenged conditions. It is used broadly to study mitochondrial function (Artal-Sanz and Tavernarakis, 2009; Palikaras , 2015; Ryu , 2016) or investigate factors mediating the switch from oxidative phosphorylation to aerobic glycolysis (Chen , 2015; Vander Heiden , 2009). In this protocol, we describe a method for the determination of oxygen consumption rates in the nematode .
摘要
氧消耗率是一个重要指标，可指示正常或代谢受到挑战情况下生物体整个生命周期中的细胞功能。它被广泛用于研究线粒体功能（阿尔塔尔 - 桑斯和塔弗纳拉基斯，2009年；帕利卡拉斯，2015年；柳，2016年）或研究介导从氧化磷酸化转变为有氧糖酵解的因素（陈，2015年；范德海登，2009年）。在本实验方案中，我们描述了一种测定线虫氧消耗率的方法。
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