Palikaras Konstantinos, Tavernarakis Nektarios
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece.
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece; Department of Basic Sciences, Faculty of Medicine, University of Crete, Crete, Greece.
Bio Protoc. 2017 Apr 5;7(7). doi: 10.21769/BioProtoc.2215.
Perturbation of mitochondrial function is a major hallmark of several pathological conditions and ageing, underlining the essential role of fine-tuned mitochondrial activity (Lopez-Otin , 2013). Mitochondrial selective autophagy, known as mitophagy, mediates the removal of dysfunctional and/or superfluous organelles, preserving cellular and organismal homeostasis (Palikaras and Tavernarakis, 2014; Pickrell and Youle, 2015; Scheibye-Knudsen , 2015). In this protocol, we describe a method for assessing mitophagy in the nematode
线粒体功能紊乱是多种病理状况和衰老的主要标志，突显了线粒体活动精细调节的重要作用（洛佩斯 - 奥廷，2013年）。线粒体选择性自噬，即线粒体自噬，介导功能失调和/或多余细胞器的清除，维持细胞和机体的稳态（帕利卡拉斯和塔弗纳拉基斯，2014年；皮克雷尔和尤尔，2015年；谢比耶 - 克努森，2015年）。在本方案中，我们描述一种评估线虫中线粒体自噬的方法
