Essel Research and Development Inc., Toronto, Canada.
ITACA, Universitat Politècnica de València, Valencia, Spain.
Sci Rep. 2017 Feb 27;7:43217. doi: 10.1038/srep43217.
Panoramic optical mapping is the primary method for imaging electrophysiological activity from the entire outer surface of Langendorff-perfused hearts. To date, it is the only method of simultaneously measuring multiple key electrophysiological parameters, such as transmembrane voltage and intracellular free calcium, at high spatial and temporal resolution. Despite the impact it has already had on the fields of cardiac arrhythmias and whole-heart computational modeling, present-day system designs precludes its adoption by the broader cardiovascular research community because of their high costs. Taking advantage of recent technological advances, we developed and validated low-cost optical mapping systems for panoramic imaging using Langendorff-perfused pig hearts, a clinically-relevant model in basic research and bioengineering. By significantly lowering financial thresholds, this powerful cardiac electrophysiology imaging modality may gain wider use in research and, even, teaching laboratories, which we substantiated using the lower-cost Langendorff-perfused rabbit heart model.
全景光学标测是从 Langendorff 灌流心脏整个外表面成像电生理活性的主要方法。迄今为止，它是唯一一种能够以高时空分辨率同时测量多个关键电生理参数（如跨膜电压和细胞内游离钙）的方法。尽管它已经对心律失常和全心计算建模领域产生了影响，但由于其成本高，目前的系统设计阻止了更广泛的心血管研究界采用它。我们利用最近的技术进步，为使用 Langendorff 灌注猪心的全景成像开发和验证了低成本的光学标测系统，这是基础研究和生物工程中一种临床相关的模型。通过显著降低财务门槛，这种强大的心脏电生理成像模式可能会在研究中得到更广泛的应用，甚至在教学实验室中得到应用，我们使用低成本的 Langendorff 灌注兔心模型证实了这一点。
