Human Physiology Lab, Moscow Institute of Physics and Technology, Moscow, Russia.
Department of Biomedical Engineering, The George Washington University, Washington DC, United States of America.
PLoS One. 2022 Mar 31;17(3):e0259174. doi: 10.1371/journal.pone.0259174. eCollection 2022.
Fluorescent imaging with voltage- or calcium-sensitive dyes, known as optical mapping, is one of the indispensable modern techniques to study cardiac or neural electrophysiology, unsurpassed by temporal and spatial resolution. High-speed CMOS cameras capable of optical registration of action potential propagation are in general very costly. We present a complete solution priced below US$1,000 (including camera and lens) at the moment of publication with an open-source image acquisition and processing software. We demonstrate that the iDS UI-3130CP rev.2 camera we used in this study is capable of 200x200 977 frames per second (FPS) action potential recordings from rodent hearts, with the signal-to-noise-ratio of a conditioned signal of 16 ± 10. A comparison with a specialized MiCAM Ultimate-L camera has shown that signal-to-noise ratio (SNR) while lower is sufficient for accurate measurements of AP waveform, conduction velocity (± 0.04 m/s) and action potential duration (± 7ms) in mouse and rat hearts. We used 4-aminopyridine to prolong the action potential duration in mouse heart, thus demonstrating that the proposed system is adequate for pharmacological studies.
使用电压或钙离子敏感染料的荧光成像，称为光学映射，是研究心脏或神经电生理学的不可或缺的现代技术之一，其时空分辨率无与伦比。能够进行动作电位传播光学记录的高速 CMOS 相机通常非常昂贵。我们目前以低于 1000 美元的价格（包括相机和镜头）提供完整的解决方案，并提供开源的图像采集和处理软件。我们证明，我们在这项研究中使用的 iDS UI-3130CP rev.2 相机能够以 200x200 的帧率记录来自啮齿动物心脏的 977 帧/秒（FPS）动作电位，条件信号的信噪比为 16±10。与专门的 MiCAM Ultimate-L 相机的比较表明，信噪比（SNR）虽然较低，但足以准确测量小鼠和大鼠心脏中的动作电位波形、传导速度（±0.04 m/s）和动作电位持续时间（±7ms）。我们使用 4-氨基吡啶延长了小鼠心脏的动作电位持续时间，从而证明了所提出的系统足以进行药理学研究。
