Podgórna K, Szczepanowicz K, Piotrowski M, Gajdošová M, Štěpánek F, Warszyński P
Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, PL-30239, Krakow, Poland.
Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, PL-30239, Krakow, Poland.
Colloids Surf B Biointerfaces. 2017 May 1;153:183-189. doi: 10.1016/j.colsurfb.2017.02.026. Epub 2017 Feb 21.
Synthesis of theranostic nanoparticles, which combine both therapeutic and diagnostic capabilities in one platform can be considered as a step forward personalized medicine, since it allows tracing the delivery of the drug to targeted organ. Thus, the aim of this work was to prepare gadolinium alginate gel nanoparticles (gadolinum nanogels - GdNG) by the reverse microemulsions and physical crosslinking method as the vehicles able to carry hydrophilic drugs and to be traced by the Magnetic Resonance Imaging (MRI). The average size of synthesized nanoparticles was about 110nm and the batch concentration was 10 particles/ml. The morphology of nanogeles was visualized by Cryo-Scanning Electron Microscopy. Surface of nanogels particles was modified by the Layer-by-Layer (LbL) technique using natural polyelectrolytes. The cytotoxicity of non-modified and LbL modified nanogels was evaluated by the cellular viability quantification and cell death assessments using MTT and LDH biochemical tests, respectively. We encapsulated the model compound - fluorescent dye (Rhodamine b) in nanogels networks and proved the possibility of GdNG visualization by MRI.
治疗诊断纳米颗粒的合成，即将治疗和诊断功能整合于一个平台，可被视为迈向个性化医疗的一步，因为它能够追踪药物向靶向器官的递送。因此，本研究的目的是通过反向微乳液和物理交联法制备海藻酸钆凝胶纳米颗粒（钆纳米凝胶-GdNG），作为能够携带亲水性药物并可通过磁共振成像（MRI）进行追踪的载体。合成纳米颗粒的平均尺寸约为110nm，批次浓度为10个颗粒/毫升。通过低温扫描电子显微镜观察纳米凝胶的形态。使用天然聚电解质通过层层（LbL）技术对纳米凝胶颗粒的表面进行修饰。分别使用MTT和LDH生化试验通过细胞活力定量和细胞死亡评估来评价未修饰和LbL修饰的纳米凝胶的细胞毒性。我们将模型化合物-荧光染料（罗丹明b）包裹在纳米凝胶网络中，并证明了通过MRI可视化GdNG的可能性。
