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一种从适合制备细菌培养基的原料中生产酵母提取物的简单快速方法。

A Simple and Rapid Protocol for Producing Yeast Extract from Suitable for Preparing Bacterial Culture Media.

作者信息

Zarei Omid, Dastmalchi Siavoush, Hamzeh-Mivehroud Maryam

机构信息

Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. ; Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran. ; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.

Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. ; Department of Medicinal Chemistry, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

出版信息

Iran J Pharm Res. 2016 Fall;15(4):907-913.

PMID:28243289
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5316271/
Abstract

Yeasts, especially are one of the oldest organisms with broad spectrum of applications, owing to their unique genetics and physiology. Yeast extract, i.e. the product of yeast cells, is extensively used as nutritional resource in bacterial culture media. The aim of this study was to develop a simple, rapid and cost benefit process to produce the yeast extract. In this procedure mechanical methods such as high temperature and pressure were utilized to produce the yeast extract. The growth of the bacteria feed with the produced yeast extract was monitored in order to assess the quality of the product. The results showed that the quality of the produced yeast extract was very promising concluded from the growth pattern of bacterial cells in media prepared from this product and was comparable with that of the three commercial yeast extracts in terms of bacterial growth properties. One of the main advantages of the current method was that no chemicals and enzymes were used, leading to the reduced production cost. The method is very simple and cost effective, and can be performed in a reasonable time making it suitable for being adopted by research laboratories. Furthermore, it can be scaled up to produce large quantities for industrial applications.

摘要

酵母,尤其是[此处原文缺失具体酵母种类],是最古老的生物之一,因其独特的遗传学和生理学特性而具有广泛的应用。酵母提取物,即酵母细胞的产物,被广泛用作细菌培养基中的营养资源。本研究的目的是开发一种简单、快速且具有成本效益的生产酵母提取物的方法。在此过程中,采用了高温和高压等机械方法来生产酵母提取物。为了评估产品质量,监测了用所生产的酵母提取物喂养的细菌的生长情况。结果表明,从用该产品制备的培养基中细菌细胞的生长模式可以得出,所生产的酵母提取物质量非常可观,并且在细菌生长特性方面与三种商业酵母提取物相当。当前方法主要优点之一是未使用化学物质和酶,从而降低了生产成本。该方法非常简单且具有成本效益,并且可以在合理时间内完成,使其适合研究实验室采用。此外,它可以扩大规模以大量生产用于工业应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21b4/5316271/470895ae9c86/ijpr-15-907-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21b4/5316271/9198559181db/ijpr-15-907-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21b4/5316271/34423aebbe9b/ijpr-15-907-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21b4/5316271/8adbec79a354/ijpr-15-907-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21b4/5316271/470895ae9c86/ijpr-15-907-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21b4/5316271/9198559181db/ijpr-15-907-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21b4/5316271/34423aebbe9b/ijpr-15-907-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21b4/5316271/8adbec79a354/ijpr-15-907-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21b4/5316271/470895ae9c86/ijpr-15-907-g004.jpg

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