Qiao Wei, Liu Quan, Li Zhipeng, Zhang Hanqing, Chen Zhuofan
Department of Oral Implantology, Guanghua School of Stomatology, Institute of Stomatological Research, Sun Yat-sen University, Hospital of Stomatology, Guangzhou, PR China; Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, PR China.
Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, PR China; Zhujiang New Town Dental Clinic, Guanghua School of Stomatology, Institute of Stomatological Research, Sun Yat-sen University, Hospital of Stomatology, Guangzhou, PR China.
Sci Technol Adv Mater. 2017 Feb 1;18(1):110-121. doi: 10.1080/14686996.2016.1263140. eCollection 2017.
As the main inorganic component of xenogenic bone graft material, bone-derived biological apatite (BAp) has been widely used in implant dentistry, oral and maxillofacial surgery and orthopedics. However, BAp produced via calcination of animal bones still suffers from some drawbacks, such as insufficient mechanical strength and inadequate degradation rate, which impede its application. Fluoride is known to play important roles in both physiological and pathological processes of human hard tissues for its double effects on bones and teeth. In order to understand the effects of fluoride on the properties of BAp, as well as the mechanism behind them, porcine bone derived hydroxyapatite (PHAp) was prepared via thermal treatment, which was then fluoride incorporated at a series concentrations of sodium fluoride, and noted as 0.25-FPHAp, 0.50-FPHAp, and 0.75-FPHAp respectively. The physicochemical characteristics of the materials, including crystal morphology, crystallinity, functional groups, elemental composition, compressive strength, porosity and solubility, were then determined. The biological properties, such as protein adsorption and cell attachment, were also evaluated. It was found that the spheroid-like crystals of PHAp were changed into rod-like after fluoride substitution, resulting in a fluoride concentration-dependent increase in compressive strength, as well as a decreased porosity and solubility of the apatite. However, even though the addition of fluoride was demonstrated to enhance protein adsorption and cell attachment of the materials, the most favorable results were intriguingly achieved in FPHAp with the least fluoride content. Collectively, low level of fluoride incorporation is proposed promising for the modification of clinically used BAp based bone substitute materials, because of its being able to maintain a good balance between physicochemical and biological properties of the apatite.
作为异种骨移植材料的主要无机成分，骨源性生物磷灰石（BAp）已广泛应用于种植牙科、口腔颌面外科和骨科。然而，通过动物骨骼煅烧产生的BAp仍存在一些缺点，如机械强度不足和降解速率不够，这阻碍了其应用。众所周知，氟化物因其对骨骼和牙齿的双重作用，在人体硬组织的生理和病理过程中发挥着重要作用。为了了解氟化物对BAp性能的影响及其背后的机制，通过热处理制备了猪骨衍生的羟基磷灰石（PHAp），然后分别用一系列浓度的氟化钠进行氟掺入，分别记为0.25-FPHAp、0.50-FPHAp和0.75-FPHAp。然后测定了材料的物理化学特性，包括晶体形态、结晶度、官能团、元素组成、抗压强度、孔隙率和溶解度。还评估了蛋白质吸附和细胞附着等生物学特性。结果发现，氟取代后PHAp的球状晶体变成了棒状，导致抗压强度随氟浓度增加，磷灰石的孔隙率和溶解度降低。然而，尽管已证明添加氟化物可增强材料的蛋白质吸附和细胞附着，但最有利的结果却令人惊讶地出现在氟含量最低的FPHAp中。总体而言，低水平的氟掺入有望用于临床使用的基于BAp的骨替代材料的改性，因为它能够在磷灰石的物理化学和生物学特性之间保持良好的平衡。
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