Flynn Gabriella E, Withers Jamie M, Macias Gerard, Sperling Justin R, Henry Sarah L, Cooper Jonathan M, Burley Glenn A, Clark Alasdair W
Biomedical Engineering Research Division, School of Engineering, University of Glasgow, Rankine Building, Oakfield Avenue, Glasgow, UK.
WestCHEM & Department of Pure & Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, G1 1XL, UK.
Chem Commun (Camb). 2017 Mar 9;53(21):3094-3097. doi: 10.1039/c7cc00288b.
We describe a new method for the immobilisation of DNA into defined patterns with sub-micron resolution, using the fluorous effect. The method is fully reversible via a simple solvent wash, allowing the patterning, regeneration and re-patterning of surfaces with no degradation in binding efficiency following multiple removal/attachment cycles of different DNA sequences.
我们描述了一种利用氟效应将DNA固定成具有亚微米分辨率的特定图案的新方法。该方法通过简单的溶剂洗涤完全可逆，允许对表面进行图案化、再生和重新图案化，在不同DNA序列的多次去除/附着循环后，结合效率不会降低。
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