Huang Fujian, Xu Huaguo, Tan Weihong, Liang Haojun
CAS Key Laboratory of Soft Matter Chemistry, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Polymer Science and Engineering, University of Science and Technology of China , Hefei, Anhui 230026, People's Republic of China.
ACS Nano. 2014 Jul 22;8(7):6849-55. doi: 10.1021/nn5024472. Epub 2014 Jul 7.
The immobilization of DNA molecules onto a solid support is a crucial step in biochip research and related applications. In this work, we report a DNA photolithography method based on photocleavage of 2-nitrobenzyl linker-modified DNA strands. These strands were subjected to ultraviolet light irradiation to generate multiple short DNA strands in a programmable manner. Coupling the toehold-mediated DNA strand-displacement reaction with DNA photolithography enabled the fabrication of a DNA chip surface with multifunctional DNA patterns having complex geometrical structures at the microscale level. The erasable DNA photolithography strategy was developed to allow different paintings on the same chip. Furthermore, the asymmetrical modification of colloidal particles was carried out by using this photolithography strategy. This strategy has broad applications in biosensors, nanodevices, and DNA-nanostructure fabrication.
将DNA分子固定到固体支持物上是生物芯片研究及相关应用中的关键步骤。在本研究中，我们报道了一种基于2-硝基苄基连接子修饰的DNA链光裂解的DNA光刻方法。这些链受到紫外线照射，以可编程的方式产生多条短DNA链。将链置换介导的DNA链置换反应与DNA光刻相结合，能够在微观尺度上制造出具有复杂几何结构的多功能DNA图案的DNA芯片表面。开发了可擦除的DNA光刻策略，以便在同一芯片上进行不同的图案绘制。此外，利用这种光刻策略对胶体颗粒进行了不对称修饰。该策略在生物传感器、纳米器件和DNA纳米结构制造中具有广泛的应用。
