Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Nat Chem Biol. 2017 May;13(5):464-466. doi: 10.1038/nchembio.2318. Epub 2017 Feb 28.
Here we report a fully automated, flow-based approach to solid-phase polypeptide synthesis, with amide bond formation in 7 seconds and total synthesis times of 40 seconds per amino acid residue. Crude peptide purities and isolated yields were comparable to those for standard-batch solid-phase peptide synthesis. At full capacity, this approach can yield tens of thousands of individual 30-mer peptides per year.
在这里，我们报告了一种完全自动化的、基于流动的固相多肽合成方法，酰胺键的形成时间为 7 秒，每个氨基酸残基的总合成时间为 40 秒。粗肽的纯度和分离收率与标准批次固相肽合成相当。在满负荷运行时，该方法每年可生产数万种 30 肽。
