Abal Paula, Louzao M Carmen, Antelo Alvaro, Alvarez Mercedes, Cagide Eva, Vilariño Natalia, Vieytes Mercedes R, Botana Luis M
Departamento de Farmacologia, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo 27002, Spain.
Laboratorio CIFGA S.A., Plaza Santo Domingo 20-5°, Lugo 27001, Spain.
Toxins (Basel). 2017 Feb 24;9(3):75. doi: 10.3390/toxins9030075.
Tetrodotoxin (TTX) is starting to appear in molluscs from the European waters and is a hazard to seafood consumers. This toxin blocks sodium channels resulting in neuromuscular paralysis and even death. As a part of the risk assessment process leading to a safe seafood level for TTX, oral toxicity data are required. In this study, a 4-level Up and Down Procedure was designed in order to determine for the first time the oral lethal dose 50 (LD) and the No Observed Adverse Effect Level (NOAEL) in mice by using an accurate well-characterized TTX standard.
河豚毒素（TTX）开始在欧洲海域的软体动物中出现，对海鲜消费者构成危害。这种毒素会阻断钠通道，导致神经肌肉麻痹甚至死亡。作为确定安全的TTX海产品限量风险评估过程的一部分，需要口服毒性数据。在本研究中，设计了一种四级上下程序，以便首次通过使用精确表征的TTX标准品来确定小鼠的半数口服致死剂量（LD）和未观察到有害作用水平（NOAEL）。
