来自根部的多糖的纯化、表征及促凝血活性
Purification, characterization and procoagulant activity of polysaccharides from roots.
作者信息
Wang Jinmei, Lian Pengli, Yu Qi, Wei Jinfeng, Kang Wen-Yi
机构信息
Institute of Chinese Materia Medica, Henan University, Kaifeng, 475004 China.
Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng, 475004 China.
出版信息
Chem Cent J. 2017 Feb 10;11:17. doi: 10.1186/s13065-017-0243-y. eCollection 2017.
Five polysaccharides, namely ADPs-1a, ADPs-1b, ADPs-2, ADPs-3a and ADPs-3b, were extracted from , purified, and identified by high performance gel permeation chromatography (HPSEC), gas chromatography (GC), Fourier transform infrared (FT-IR) spectrometer and nuclear magnetic resonance spectra (NMR), including the determination of procoagulant activity in vitro. The average molecular weight () of the polysaccharides was 153,800, 8312, 111,700, 3766 and 96,680 g/mol, respectively. Coagulation assays indicated that ADPs-1b, ADPs-2, ADPs-3a and ADPs-3b had procoagulant activities. ADPs-1b exerted the procoagulant activities through intrinsic pathway, extrinsic pathway and increased the content of FIB in vitro. ADPs-2 exerted the procoagulant activities through intrinsic pathway and extrinsic pathway. ADPs-3a had procoagulant activities and the activity was associated with the intrinsic pathway and increased the content of FIB. ADPs-3b exerted the activities through extrinsic pathway and increased the content of FIB.
从……中提取了五种多糖，即ADPs-1a、ADPs-1b、ADPs-2、ADPs-3a和ADPs-3b，进行了纯化，并通过高效凝胶渗透色谱法（HPSEC）、气相色谱法（GC）、傅里叶变换红外光谱仪（FT-IR）和核磁共振光谱（NMR）进行了鉴定，包括体外促凝血活性的测定。这些多糖的平均分子量（）分别为153,800、8312、111,700、3766和96,680 g/mol。凝血试验表明，ADPs-1b、ADPs-2、ADPs-3a和ADPs-3b具有促凝血活性。ADPs-1b通过内源性途径、外源性途径发挥促凝血活性，并在体外增加纤维蛋白原（FIB）的含量。ADPs-2通过内源性途径和外源性途径发挥促凝血活性。ADPs-3a具有促凝血活性，且该活性与内源性途径有关，并增加了FIB的含量。ADPs-3b通过外源性途径发挥活性并增加了FIB的含量。
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