Yang Lan, Li Qian, Feng Yanmei, Qiu Daiyu
Gansu Provincial Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.
J Anal Methods Chem. 2020 Mar 24;2020:8987560. doi: 10.1155/2020/8987560. eCollection 2020.
In this study, a quantitative H NMR method (H-qNMR) for determining the contents of imperatorin, byakangelicin, and oxypeucedanin in in traditional Chinese medicine (TCM) has been established. Dried plant material was extracted exhaustively with methanol by an ultrasonication-assisted extraction method. The H-qNMR measurements were performed on a 600 -MHz spectrometer with hydroquinone as the internal standard reference in deuterated dimethyl sulfoxide (DMSO-d) solvent. Quantification was carried out using the H resonance signals at 6.55 ppm for hydroquinone and 7.68, 7.38-7.39, and 6.38-6.39 ppm for imperatorin, byakangelicin, and oxypeucedanin, respectively. The linearity, limit of detection (LOD), limit of quantitation (LOQ), precision, reproducibility, stability, and recovery of the methodology were evaluated, and results were good. The newly developed method has been applied to determine the three coumarins in .
在本研究中，已建立一种用于测定中药中欧前胡素、白芷醚和氧化前胡素含量的定量核磁共振氢谱方法（H-qNMR）。采用超声辅助提取法用甲醇对干燥的植物材料进行彻底提取。在600兆赫兹的光谱仪上，以对苯二酚为内标物，在氘代二甲基亚砜（DMSO-d）溶剂中进行H-qNMR测量。分别使用对苯二酚在6.55 ppm处的氢共振信号以及欧前胡素、白芷醚和氧化前胡素在7.68、7.38 - 7.39和6.38 - 6.39 ppm处的氢共振信号进行定量分析。对该方法的线性、检测限（LOD）、定量限（LOQ）、精密度、重现性、稳定性和回收率进行了评估，结果良好。新开发的方法已应用于测定[具体中药名称未给出]中的这三种香豆素。
