Li Ying-Hong, Zhou Bei-Lei, Qian Ming-Rong, Wang Qiang, Zhang Hu
Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China.
Institute of Quality and Standard for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
J Anal Methods Chem. 2017;2017:6075405. doi: 10.1155/2017/6075405. Epub 2017 Jan 26.
Carbendazim is usually used to control the of rapes during the flowering period. This paper presents a study on transfer assessment of carbendazim residues from rape flowers to apicultural products. In the field trials, the rapes were sprayed with carbendazim on standard dosage. Bees produced apicultural products (bee pollen, honey, and royal jelly) from sprayed rapes. Apicultural products were collected on a regular basis. Carbendazim residues were extracted from bee pollen, honey, and royal jelly, respectively. HPLC/ESI-MS/MS method was developed and partially validated to identify and quantify carbendazim residues. The limits of quantification in pollen, honey, and royal jelly were 0.01 mg/kg. Mathematical curve fitting was carried out on the basis of transfer assessment of carbendazim residues from rape flowers to apicultural products. The respective carbendazim residues were 1.10 ± 0.03 mg/kg in pollen on 18th day, 0.032 ± 0.001 mg/kg in honey on 24th day, and 0.077 ± 0.002 mg/kg in royal jelly on 22nd day. Transfer assessment and mathematical curve fitting of carbendazim residues from rape flowers to apicultural products show carbendazim diminished over spraying time. The gap of carbendazim residues between pollen and honey is decreased with time. The carbendazim residues in pollen are 10 times higher than that of honey and jelly.
多菌灵通常用于在花期防治油菜病虫害。本文介绍了一项关于多菌灵残留从油菜花向蜂产品转移评估的研究。在田间试验中，按照标准剂量给油菜喷洒多菌灵。蜜蜂从喷洒过多菌灵的油菜花中生产蜂产品（蜂花粉、蜂蜜和蜂王浆）。定期收集蜂产品。分别从蜂花粉、蜂蜜和蜂王浆中提取多菌灵残留。开发并部分验证了HPLC/ESI-MS/MS方法，用于鉴定和定量多菌灵残留。花粉、蜂蜜和蜂王浆中的定量限均为0.01 mg/kg。基于多菌灵残留从油菜花向蜂产品的转移评估进行了数学曲线拟合。第18天花粉中的多菌灵残留量为1.10±0.03 mg/kg，第24天蜂蜜中的为0.032±0.001 mg/kg，第22天蜂王浆中的为0.077±0.002 mg/kg。多菌灵残留从油菜花向蜂产品的转移评估和数学曲线拟合表明，随着喷洒时间的推移，多菌灵残留量减少。花粉和蜂蜜中多菌灵残留量的差距随时间减小。花粉中的多菌灵残留量比蜂蜜和蜂王浆中的高10倍。
