Baraúna Rafael A, Freitas Dhara Y, Pinheiro Juliana C, Folador Adriana R C, Silva Artur
Laboratory of Genomics and Bioinformatics, Center of Genomics and Systems Biology, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, Brazil.
Proteomes. 2017 Feb 23;5(1):9. doi: 10.3390/proteomes5010009.
Since the publication of one of the first studies using 2D gel electrophoresis by Patrick H. O'Farrell in 1975, several other studies have used that method to evaluate cellular responses to different physicochemical variations. In environmental microbiology, bacterial adaptation to cold environments is a "hot topic" because of its application in biotechnological processes. As in other fields, gel-based and gel-free proteomic methods have been used to determine the molecular mechanisms of adaptation to cold of several psychrotrophic and psychrophilic bacterial species. In this review, we aim to describe and discuss these main molecular mechanisms of cold adaptation, referencing proteomic studies that have made significant contributions to our current knowledge in the area. Furthermore, we use B7 as a model organism to present the importance of integrating genomic, transcriptomic, and proteomic data. This species has been isolated in Antarctica and previously studied at all three omic levels. The integration of these data permitted more robust conclusions about the mechanisms of bacterial adaptation to cold.
自1975年帕特里克·H·奥法雷尔发表了首批使用二维凝胶电泳的研究之一以来，其他一些研究也采用该方法来评估细胞对不同物理化学变化的反应。在环境微生物学中，细菌对寒冷环境的适应是一个“热门话题”，因为它在生物技术过程中有应用。与其他领域一样，基于凝胶和无凝胶的蛋白质组学方法已被用于确定几种嗜冷和嗜冷细菌物种适应寒冷的分子机制。在这篇综述中，我们旨在描述和讨论这些主要的冷适应分子机制，并参考对我们目前在该领域的知识有重大贡献的蛋白质组学研究。此外，我们以B7作为模式生物来阐述整合基因组、转录组和蛋白质组数据的重要性。该物种已在南极洲分离出来，之前已在所有三个组学水平上进行过研究。这些数据的整合使得关于细菌适应寒冷机制的结论更加可靠。
