Division of Polar Life Science, Korea Polar Research Institute, Incheon, 21990, Republic of Korea.
Department of Molecular Bioscience, Kangwon National University, Chuncheon, 24341, Republic of Korea.
J Microbiol. 2023 Jul;61(7):663-672. doi: 10.1007/s12275-023-00068-y. Epub 2023 Aug 24.
Subtercola boreus K300 is a novel psychrophilic strain that was isolated from permanently cold groundwater in Finland and has also been found in several places in Antarctica including lake, soil, and rocks. We performed genomic and transcriptomic analyses of 5 strains from Antarctica and a type strain to understand their adaptation to different environments. Interestingly, the isolates from rocks showed a low growth rate and smaller genome size than strains from the other isolation sources (lake, soil, and groundwater). Based on these habitat-dependent characteristics, the strains could be classified into two ecotypes, which showed differences in energy production, signal transduction, and transcription in the clusters of orthologous groups of proteins (COGs) functional category. In addition, expression pattern changes revealed differences in metabolic processes, including uric acid metabolism, DNA repair, major facilitator superfamily (MFS) transporters, and xylose degradation, depending on the nutritional status of their habitats. These findings provide crucial insights into the environmental adaptation of bacteria, highlighting genetic diversity and regulatory mechanisms that enable them to thrive in the cryosphere.
北极海底冷杆菌 K300 是一种新型的嗜冷菌株，它从芬兰的永久性冷地下水中分离出来，也在南极洲的多个地方（包括湖泊、土壤和岩石）被发现。我们对来自南极洲的 5 株菌株和一株模式菌株进行了基因组和转录组分析，以了解它们对不同环境的适应。有趣的是，与来自其他分离源（湖泊、土壤和地下水）的菌株相比，来自岩石的分离物的生长速度较慢，基因组大小较小。基于这些与栖息地相关的特征，这些菌株可以分为两种生态型，它们在蛋白质直系同源簇（COG）功能类别中的能量产生、信号转导和转录方面表现出差异。此外，表达模式的变化揭示了代谢过程的差异，包括尿酸代谢、DNA 修复、主要易化因子超家族（MFS）转运蛋白和木糖降解，这取决于它们栖息地的营养状况。这些发现为细菌的环境适应提供了重要的见解，突出了遗传多样性和调控机制，使它们能够在冰冻圈中茁壮成长。
