Offit P A, Dudzik K I
Department of Medical Microbiology, Stanford University School of Medicine, California 94305.
J Virol. 1988 Jan;62(1):127-31. doi: 10.1128/JVI.62.1.127-131.1988.
Splenocytes from adult C57BL6 (H-2b) mice orally inoculated with nonmurine rotaviruses lysed syngeneic rotavirus-infected target cells. Cytotoxic T lymphocytes (CTLs) were responsible for this cytotoxic activity. Cytotoxic activity was (i) detected 7 days after primary oral inoculation; (ii) not detected in uninoculated animals; (iii) specific for rotavirus-infected target cells; (iv) eliminated by treatment with Thy 1.2-specific immunoglobulin M and complement; and (v) restricted at H-2Db. In addition, rotavirus-specific CTLs cross-reacted with target cells infected with different human or animal rotavirus serotypes. Heterotypic protection against rotavirus challenge may be mediated by cross-reactive CTLs.
用非鼠类轮状病毒经口接种的成年C57BL6（H-2b）小鼠的脾细胞可裂解同基因的轮状病毒感染的靶细胞。细胞毒性T淋巴细胞（CTL）负责这种细胞毒性活性。细胞毒性活性表现为：（i）初次经口接种7天后可检测到；（ii）未接种的动物中未检测到；（iii）对轮状病毒感染的靶细胞具有特异性；（iv）用Thy 1.2特异性免疫球蛋白M和补体处理后消除；（v）受H-2Db限制。此外，轮状病毒特异性CTL与感染不同人或动物轮状病毒血清型的靶细胞发生交叉反应。针对轮状病毒攻击的异型保护可能由交叉反应性CTL介导。
