Stoye J P, Coffin J M
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111.
J Virol. 1988 Jan;62(1):168-75. doi: 10.1128/JVI.62.1.168-175.1988.
Inbred mice contain three classes of endogenous nonecotropic murine leukemia virus-related sequences, namely xenotropic, polytropic, and modified polytropic proviruses. Oligonucleotide probes specific for the three different classes were prepared and used to examine the diversity of endogenous sequences present in eight different strains of mice: HRS/J, BALB/cJ, A/J, AKR/J, C57BL/6J, DBA/2J, C57L/J, and C3H/HeJ. A high degree of polymorphism was observed. Overall, the strains showed between 17% (A/J and HRS/J) and 65% (C57BL/6J and C57L/J) shared proviruses, and only four proviruses were present in all eight strains. The similarity among the strains is due in part to the few proviruses present in all of the strains but also represents the independent assortment of a limited set of proviruses. These oligonucleotides provide a basis for determining the stability, distribution, and mutagenic potential of nonecotropic proviruses within the mouse genome.
近交系小鼠含有三类内源性非嗜亲性鼠白血病病毒相关序列，即嗜异性、多嗜性和修饰多嗜性前病毒。制备了针对这三种不同类型的寡核苷酸探针，并用于检测八种不同品系小鼠（HRS/J、BALB/cJ、A/J、AKR/J、C57BL/6J、DBA/2J、C57L/J和C3H/HeJ）中存在的内源性序列的多样性。观察到高度的多态性。总体而言，这些品系显示出17%（A/J和HRS/J）至65%（C57BL/6J和C57L/J）的共享前病毒，并且所有八个品系中仅存在四种前病毒。品系之间的相似性部分归因于所有品系中存在的少数前病毒，但也代表了一组有限前病毒的独立分类。这些寡核苷酸为确定非嗜亲性前病毒在小鼠基因组中的稳定性、分布和诱变潜力提供了基础。
