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Determination of the single polyadenylation site of the human pro alpha 1(II) collagen gene.

作者信息

Elima K, Vuorio T, Vuorio E

机构信息

Department of Medical Biochemistry, University of Turku, Finland.

出版信息

Nucleic Acids Res. 1987 Nov 25;15(22):9499-504. doi: 10.1093/nar/15.22.9499.

DOI:10.1093/nar/15.22.9499
PMID:2825137
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC306483/
Abstract

Several cDNA clones for the human type II procollagen mRNA were isolated from a cartilage cDNA library. Six of the clones containing the longest inserts were subjected to restriction site mapping for alignment. All these six clones extended to the poly A tail. The longest clone, containing a 1470 bp insert, was named pHCAR3. Sequencing of pHCAR3 made it clear that neither of the two canonical AATAAA sequences of the human type II collagen gene is used as the polyadenylation signal. Two 60 bp stretches of high interspecies homology terminating in a hexanucleotide ATTAAA, located 23 nucleotides upstream of the poly A tail, apparently have an important role in determining the single polyadenylation signal for this gene. S1 protection experiments confirmed these observations.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b988/306483/4e2e6aae74db/nar00266-0394-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b988/306483/4e2e6aae74db/nar00266-0394-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b988/306483/4e2e6aae74db/nar00266-0394-a.jpg

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引用本文的文献

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本文引用的文献

1
Sequence determination and analysis of the 3' region of chicken pro-alpha 1(I) and pro-alpha 2(I) collagen messenger ribonucleic acids including the carboxy-terminal propeptide sequences.鸡原α1（I）和原α2（I）型胶原信使核糖核酸3'区域的序列测定与分析，包括羧基末端前肽序列。
Biochemistry. 1981 Feb 17;20(4):996-1006. doi: 10.1021/bi00507a054.
2
Identification of messenger RNA for human type II collagen.
FEBS Lett. 1984 Sep 3;174(2):238-42. doi: 10.1016/0014-5793(84)81165-5.
3
Structure and sequence of the chicken type II procollagen gene. Characterization of the region encoding the carboxyl-terminal telopeptide and propeptide.鸡Ⅱ型原胶原基因的结构与序列。编码羧基末端端肽和前肽区域的特征分析。
多功能蛋白聚糖基因在人关节软骨中的表达及与聚集蛋白聚糖的mRNA剪接变异比较。
Biochem J. 1993 Apr 15;291 ( Pt 2)(Pt 2):361-7. doi: 10.1042/bj2910361.
4
Co-expression of collagens II and XI and alternative splicing of exon 2 of collagen II in several developing human tissues.胶原蛋白II和XI在几种发育中的人体组织中的共表达以及胶原蛋白II外显子2的可变剪接。
Biochem J. 1993 Sep 1;294 ( Pt 2)(Pt 2):595-602. doi: 10.1042/bj2940595.
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Biochem J. 1993 Sep 1;294 ( Pt 2)(Pt 2):365-71. doi: 10.1042/bj2940365.
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J Clin Invest. 1993 Mar;91(3):829-37. doi: 10.1172/JCI116303.
8
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Biochem J. 1989 Sep 1;262(2):521-8. doi: 10.1042/bj2620521.
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Expression of mRNAs coding for the alpha 1 chain of type XIII collagen in human fetal tissues: comparison with expression of mRNAs for collagen types I, II, and III.编码 XIII 型胶原α1 链的 mRNA 在人胎儿组织中的表达：与 I、II 和 III 型胶原 mRNA 表达的比较。
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