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编码小牛II型胶原蛋白原α1链的cDNA和基因组克隆的分析。

Analysis of cDNA and genomic clones coding for the pro alpha 1 chain of calf type II collagen.

作者信息

Sangiorgi F O, Benson-Chanda V, de Wet W J, Sobel M E, Ramirez F

出版信息

Nucleic Acids Res. 1985 Apr 25;13(8):2815-26. doi: 10.1093/nar/13.8.2815.

DOI:10.1093/nar/13.8.2815
PMID:2582365
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC341196/
Abstract

A bovine cDNA library constructed from fetal cartilage RNA was screened with a pro alpha 1(II) collagen specific chicken cDNA. A recombinant clone (Bc 7), with an insert of 1 kb, was identified and shown to contain sequences exhibiting 85% homology with the chicken pro alpha 1(II) collagen C-propeptide. Interspecies comparison strongly suggested that one potential glycosylation site present in the avian C-propeptide is not utilized, since this site is absent in the bovine chain. In addition, two overlapping genomic clones (Pal 3 and Pal 4) were isolated and partially characterized. These clones span 23 kb of DNA and contain approximately 17 kb of the pro alpha 1(II) calf gene. Sequencing of exon 1 has determined the length of the 3' untranslated region and the exact location of the polyadenylation attachment site.

摘要

用鸡的原α1（II）型胶原特异性cDNA筛选由胎儿软骨RNA构建的牛cDNA文库。鉴定出一个插入片段为1 kb的重组克隆（Bc 7），并显示其包含与鸡原α1（II）型胶原C-前肽具有85%同源性的序列。种间比较强烈表明，禽类C-前肽中存在的一个潜在糖基化位点未被利用，因为该位点在牛链中不存在。此外，分离出两个重叠的基因组克隆（Pal 3和Pal 4）并进行了部分特征分析。这些克隆跨越23 kb的DNA，包含约17 kb的小牛原α1（II）型基因。外显子1的测序确定了3'非翻译区的长度和聚腺苷酸化附着位点的确切位置。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af08/341196/6925399abb2f/nar00302-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af08/341196/e09eed942186/nar00302-0147-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af08/341196/b38fc3dae338/nar00302-0147-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af08/341196/6925399abb2f/nar00302-0152-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af08/341196/e09eed942186/nar00302-0147-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af08/341196/b38fc3dae338/nar00302-0147-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/af08/341196/6925399abb2f/nar00302-0152-a.jpg

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本文引用的文献

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Primary structure of the carbohydrate-containing regions of the carboxyl propeptides of type I procollagen.I型前胶原羧基端前肽含糖区域的一级结构。
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Isolation of cDNA clones for the human complement protein factor B, a class III major histocompatibility complex gene product.
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Chondrocalcin is identical with the C-propeptide of type II procollagen.软骨钙素与II型前胶原的C-前肽相同。
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Nonexpression of cartilage type II collagen in a case of Langer-Saldino achondrogenesis.1型朗格-萨尔迪诺软骨发育不全病例中软骨II型胶原蛋白的无表达
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The pro alpha 2(V) collagen gene is evolutionarily related to the major fibrillar-forming collagens.原α2（V）型胶原蛋白基因在进化上与主要的形成纤维的胶原蛋白相关。
Nucleic Acids Res. 1987 Jan 12;15(1):181-98. doi: 10.1093/nar/15.1.181.
8
Exon/intron organization of the chicken type II procollagen gene: intron size distribution suggests a minimal intron size.鸡Ⅱ型前胶原基因的外显子/内含子结构：内含子大小分布表明存在最小内含子大小。
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2325-9. doi: 10.1073/pnas.83.8.2325.
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Large introns in the 3' end of the gene for the pro alpha 1 (IV) chain of human basement membrane collagen.人基底膜胶原蛋白原α1（IV）链基因3'端的大内含子。
Proc Natl Acad Sci U S A. 1986 Mar;83(6):1568-72. doi: 10.1073/pnas.83.6.1568.
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Determination of the single polyadenylation site of the human pro alpha 1(II) collagen gene.
Nucleic Acids Res. 1987 Nov 25;15(22):9499-504. doi: 10.1093/nar/15.22.9499.
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A conserved nucleotide sequence, coding for a segment of the C-propeptide, is found at the same location in different collagen genes.在不同的胶原蛋白基因中，编码C-前肽片段的保守核苷酸序列位于相同位置。
Nucleic Acids Res. 1983 May 11;11(9):2733-44. doi: 10.1093/nar/11.9.2733.
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Isolation and characterization of genomic clones corresponding to the human type II procollagen gene.与人类II型前胶原基因对应的基因组克隆的分离与鉴定。
Nucleic Acids Res. 1984 Jan 25;12(2):1025-38. doi: 10.1093/nar/12.2.1025.
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Identification of genomic DNA coding for chicken type II procollagen.鸡Ⅱ型原胶原编码基因组DNA的鉴定。
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Construction and characterization of type II collagen complementary deoxyribonucleic acid clones.II型胶原蛋白互补脱氧核糖核酸克隆的构建与特性分析
Nucleic Acids Res. 1983 Sep 10;11(17):6021-39. doi: 10.1093/nar/11.17.6021.