Department of Electrical Engineering, Indian Institute of Technology Bombay, Mumbai, 400076, India.
BioPRIA, Chemical Engineering department, Monash University, Clayton VIC 3800, Australia.
Sci Rep. 2017 Mar 2;7:43905. doi: 10.1038/srep43905.
Distinguishing a specific biomarker from a biofluid sample containing a large variety of proteins often requires the selective preconcentration of that particular biomarker to a detectable level for analysis. Low-cost, paper-based device is an emerging opportunity in diagnostics. In the present study, we report a novel Zinc oxide nanorods functionalized paper platform for the preconcentration of Myoglobin, a cardiac biomarker. Zinc oxide nanorods were grown on a Whatman filter paper no. 1 via the standard hydrothermal route. The growth of Zinc oxide nanorods on paper was confirmed by a combination of techniques consisting of X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS,) scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDX) analysis. The Zinc oxide nanorods modified Whatman filter paper (ZnO-NRs/WFP) was further tested for use as a protein preconcentrator. Paper-based ELISA was performed for determination of pre-concentration of cardiac marker protein Myoglobin using the new ZnO-NRs/WFP platform. The ZnO-NRs/WFP could efficiently capture the biomarker even from a very dilute solution (Myoglobin < 50 nM). Our ELISA results show a threefold enhancement in protein capture with ZnO-NRs/WFP compared to unmodified Whatman filter paper, allowing accurate protein analysis and showing the diagnostic concept.
从包含多种蛋白质的生物流体样本中鉴别特定的生物标志物，通常需要将该特定生物标志物选择性地浓缩到可检测水平进行分析。低成本、基于纸张的设备是诊断领域的一个新兴机会。在本研究中，我们报告了一种用于肌红蛋白（一种心脏标志物）预浓缩的新型氧化锌纳米棒功能化纸张平台。氧化锌纳米棒通过标准水热法在 Whatman 滤纸上 1 号上生长。通过 X 射线衍射（XRD）、X 射线光电子能谱（XPS）、扫描电子显微镜（SEM）和能谱分析（EDX）等技术的组合，证实了氧化锌纳米棒在纸上的生长。进一步测试了修饰有氧化锌纳米棒的 Whatman 滤纸（ZnO-NRs/WFP）作为蛋白质预浓缩器的用途。使用新的 ZnO-NRs/WFP 平台进行基于纸张的 ELISA 以测定心脏标志物蛋白肌红蛋白的预浓缩。即使在非常稀释的溶液中（肌红蛋白<50nM），ZnO-NRs/WFP 也能有效地捕获生物标志物。与未修饰的 Whatman 滤纸相比，我们的 ELISA 结果显示 ZnO-NRs/WFP 可使蛋白质捕获能力提高三倍，从而能够进行准确的蛋白质分析并展示诊断概念。
