Zanjan Pharmaceutical Nanotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Medicinal Chemistry, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
Mater Sci Eng C Mater Biol Appl. 2017 May 1;74:1-9. doi: 10.1016/j.msec.2016.12.023. Epub 2016 Dec 9.
In this study, PEGylated multiwall carbon nanotubes (MWNTs)-based drug delivery system was developed. Ibuprofen as a model drug was loaded by physical and chemical method. The surface functionalization of nanotubes was carried out by enrichment of acylated groups. In order to synthesis PEGylated MWNTs, hydrophilic diamino-polyethylene glycol was covalently linked to the MWNTs surface via amidation reaction. Finally, ibuprofen was chemically and physically loaded on the PEGylated MWNTs. The resultants were characterized by FTIR, AFM, and DLS techniques. Cytotoxicity of PEGylated MWNTs were examined by MTT assay and the results revealed that PEG functionalized nanotubes did not show significant detrimental effects on the viability of L929 Cells. The percent of drug loading for chemically and physically drug payload carrier were determined to be 52.5% and 38%, respectively. The release of ibuprofen from covalently conjugated and non-covalent drug loaded PEGylated MWNTs at pH=7.4, and 5.3 were investigated, as well. From the results, it was found that chemically loaded MWNTs showed much sustained release behavior compared to the physically loaded one, especially at pH=5.3. The kinetic of drug release was also investigated. The results strongly suggest that the chemically conjugated PEGylated MWNTs could be used as controlled release system for various drugs.
在这项研究中，开发了基于聚乙二醇化多壁碳纳米管（MWNTs）的药物传递系统。布洛芬作为模型药物，通过物理和化学方法进行负载。通过酰化基团的富集对纳米管进行表面功能化。为了合成聚乙二醇化 MWNTs，亲水性二氨基聚乙二醇通过酰胺化反应共价连接到 MWNTs 表面。最后，将布洛芬通过化学和物理方法负载在聚乙二醇化 MWNTs 上。通过傅里叶变换红外光谱（FTIR）、原子力显微镜（AFM）和动态光散射（DLS）技术对产物进行了表征。通过 MTT 测定法研究了聚乙二醇化 MWNTs 的细胞毒性，结果表明 PEG 官能化的纳米管对 L929 细胞的活力没有显著的不利影响。化学和物理载药载体的载药百分率分别为 52.5%和 38%。还研究了在 pH=7.4 和 5.3 时共价结合和非共价载药的聚乙二醇化 MWNTs 中布洛芬的释放情况。结果表明，与物理负载相比，化学负载的 MWNTs 表现出更持久的释放行为，尤其是在 pH=5.3 时。还研究了药物释放的动力学。结果强烈表明，化学接枝的聚乙二醇化 MWNTs 可作为各种药物的控释系统。
