Deus Daniela, Krischek Carsten, Pfeifer Yvonne, Sharifi Ahmad Reza, Fiegen Ulrike, Reich Felix, Klein Guenter, Kehrenberg Corinna
Institute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Robert Koch-Institute, Nosocomial Pathogens and Antibiotic Resistance, Wernigerode, Germany.
Diagn Microbiol Infect Dis. 2017 May;88(1):88-92. doi: 10.1016/j.diagmicrobio.2017.01.023. Epub 2017 Feb 8.
A total of 174 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates collected from humans (n=140) and healthy broiler chickens (n = 34) was included in the study. The MIC values of alkyl diaminoethyl glycin hydrochloride, benzethonium chloride, benzalkonium chloride, chlorhexidine, acriflavine, copper sulfate, silver nitrate and zinc chloride were determined by the broth microdilution method. Significant differences in MIC distributions were found between human and avian isolates and between CTX-M-, SHV- and TEM-type ESBL E. coli for chlorhexidine, silver nitrate, zinc chloride and copper sulfate by statistical analysis. Isolates with reduced susceptibility were investigated for the presence and localization of tolerance-mediating genes by PCR analysis and Southern blotting. The genes emrE, mdfA, sugE(c), cueO, copA, zntA and zitB were commonly present in isolates with elevated MICs, while the genes qacE∆1, qacF, qacH, sugE(p), cusC and pcoA, were less prevalent. In several isolates, a plasmid localization of the genes qacE∆1, qacF, qacH and sugE(p) on large plasmids >20 kb was detected.
本研究纳入了从人类（n = 140）和健康肉鸡（n = 34）中收集的总共174株产超广谱β-内酰胺酶（ESBL）的大肠杆菌分离株。采用肉汤微量稀释法测定盐酸烷基二氨基乙基甘氨酸、苄索氯铵、苯扎氯铵、氯己定、吖啶黄、硫酸铜、硝酸银和氯化锌的最低抑菌浓度（MIC）值。通过统计分析发现，人类和禽类分离株之间以及CTX-M型、SHV型和TEM型ESBL大肠杆菌之间，在氯己定、硝酸银、氯化锌和硫酸铜的MIC分布上存在显著差异。通过聚合酶链反应（PCR）分析和Southern印迹法，对药敏性降低的分离株进行耐受介导基因的存在和定位研究。emrE、mdfA、sugE(c)、cueO、copA、zntA和zitB基因在MIC升高的分离株中普遍存在，而qacE∆1、qacF、qacH、sugE(p)、cusC和pcoA基因则较少见。在几株分离株中，检测到qacE∆1、qacF、qacH和sugE(p)基因在大于20 kb的大质粒上呈质粒定位。
