土耳其人群中伴或不伴有糖尿病足溃疡的2型糖尿病患者白细胞介素-6 -174 G>C基因多态性的关系

The relationship of Interleukin-6 -174 G>C gene polymorphism in type 2 diabetic patients with and without diabetic foot ulcers in Turkish population.

作者信息

Erdogan Mehmet, Kulaksizoglu Mustafa, Solmaz Soner, Berdeli Afig

机构信息

Ege University Medical School, Department of Endocrinology and Metabolic Diseases, Bornova, 35100 Izmir, Turkey.

Necmettin Erbakan University Medical School, Department of Endocrinology and Metabolic Diseases, Konya, Turkey.

出版信息

Foot (Edinb). 2017 Mar;30:27-31. doi: 10.1016/j.foot.2017.02.001. Epub 2017 Feb 10.

DOI:10.1016/j.foot.2017.02.001

PMID:28257947

Abstract

OBJECTIVE

We aims investigate Turkish type 2 diabetic patients with/without diabetic foot ulcers and healthy group and examined the contribution of Interleukin (IL)-6 -174 G>C gene polymorphism to the development of diabetic foot ulcers.

DESIGN AND PATIENTS

The Interleukin (IL)-6 -174 G>C genotypes were determined prospectively in 50 patients with diabetic foot ulcers and 35 without diabetic foot ulcers and a control group of 119 healthy individuals. Genotyping of the Interleukin (IL)-6 -174 G>C gene polymorphisms for all individuals was performed by PCR-RFLP method.

RESULTS

The genotype IL6 distribution did differ between the control group (CC 13.3%, GC 66.7%, GG 20%) and type 2 diabetic patients (CC 2.4%, GC 47.1%, GG 50.6%) (P<0.001). The genotype IL6 distribution did not differ between type 2 diabetic patients group (CC 0%, GC 45.7%, GG 54.3%) and diabetic foot ulcers (CC 4%, GC 48%, 48%) (P>0.05). The frequency of the polymorphic G allele in between the control group and type 2 diabetic patients was no similar for the groups (58.4% and 74.1%, respectively) (p<0.05). The frequency of the polymorphic G allele in between the type 2 diabetic patients and diabetic foot ulcers was similar for the groups (77.1% and 72%, respectively) (p>0.05).

CONCLUSION

The gene polymorphism of Interleukin-6 -174 G>C and G allele are an risk factor for diabetes, but gene polymorphism of Interleukin-6 -174 G>C is not an independent risk factor for diabetic foot. Genetic factors in the pathogenesis of diabetic foot may also show any changes in different populations.

摘要

目的

我们旨在研究患有/未患有糖尿病足溃疡的土耳其2型糖尿病患者以及健康组，并探讨白细胞介素（IL）-6 -174 G>C基因多态性对糖尿病足溃疡发生发展的影响。

设计与患者

前瞻性地确定了50例糖尿病足溃疡患者、35例无糖尿病足溃疡患者以及119名健康个体组成的对照组中白细胞介素（IL）-6 -174 G>C基因型。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对所有个体的白细胞介素（IL）-6 -174 G>C基因多态性进行基因分型。

结果

对照组（CC 13.3%，GC 66.7%，GG 20%）与2型糖尿病患者（CC 2.4%，GC 47.1%，GG 50.6%）之间的IL6基因型分布存在差异（P<0.001）。2型糖尿病患者组（CC 0%，GC 45.7%，GG 54.3%）与糖尿病足溃疡组（CC 4%，GC 48%，48%）之间的IL6基因型分布无差异（P>0.05）。对照组与2型糖尿病患者组之间多态性G等位基因的频率不相似（分别为58.4%和74.1%）（p<0.05）。2型糖尿病患者组与糖尿病足溃疡组之间多态性G等位基因的频率相似（分别为77.1%和72%）（p>0.05）。