Hong So-Hye, Li Mei, Jeung Eui-Bae, Lee Geun-Shik, Hong Eui-Ju, Choi Young-Whan, An Beum-Soo
Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University, Miryang‑si, Gyeongsangnam‑do, Republic of Korea.
Department of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang-si, Gyeongsangnam‑do, Republic of Korea.
Int J Oncol. 2017 Apr;50(4):1448-1454. doi: 10.3892/ijo.2017.3881. Epub 2017 Feb 17.
Prolactin (PRL) is secreted from the pituitary gland in response to eating, mating, and ovulation. Increased serum concentration of PRL during pregnancy contributes to enlargement of the mammary glands of the breasts and prepares for production of milk. However, high PRL levels derived from prolactinoma and hyperprolactinemia induce physiological disorders such as infertility and early menopause. Natural compounds isolated from S. chinensis have been known to possess anti-oxidative, anti-inflammatory and anti-diabetic effects. In the present study, we examined the therapeutic effect of S. chinensis and its single compounds on hyperprolactinemia in the pituitary gland. In rat pituitary cells, PRL expression levels were examined using real-time PCR and western blot assay. Crude S. chinensis extract and its single compound, gomisin N, reduced mRNA and protein levels of PRL in GH3 cells. In addition, cell proliferation and PRL target gene expression in cells were modulated by S. chinensis. Similar to the in vitro experiments, crude S. chinensis extract and gomisin N reduced PRL levels in the pituitary and serum of immature female rats. These results show that S. chinensis and its single compound, gomisin N, are regulators of PRL production and may be candidates for treatment of hyperprolactinemia and prolactinoma.
催乳素（PRL）是垂体在进食、交配和排卵时分泌的。孕期血清催乳素浓度升高有助于乳房乳腺增大并为乳汁分泌做准备。然而，源自催乳素瘤和高催乳素血症的高催乳素水平会引发诸如不孕和早绝经等生理紊乱。已知从中华五味子中分离出的天然化合物具有抗氧化、抗炎和抗糖尿病作用。在本研究中，我们检测了中华五味子及其单一化合物对垂体高催乳素血症的治疗效果。在大鼠垂体细胞中，使用实时PCR和蛋白质印迹分析法检测催乳素表达水平。中华五味子粗提物及其单一化合物戈米辛N降低了GH3细胞中催乳素的mRNA和蛋白质水平。此外，中华五味子调节了细胞增殖和细胞中催乳素靶基因的表达。与体外实验相似，中华五味子粗提物和戈米辛N降低了未成熟雌性大鼠垂体和血清中的催乳素水平。这些结果表明，中华五味子及其单一化合物戈米辛N是催乳素产生的调节剂，可能是治疗高催乳素血症和催乳素瘤的候选药物。
