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五味子果实提取物的生物矿化分离、浓缩及效果评价。

Biomineralized separation, concentration, and evaluation of the effectiveness of Schisandra chinensis fruit extract.

机构信息

Department of Food Biotechnology, Dong-A University, Busan 49315, Republic of Korea.

GeneCellPharm Corporation, Seoul 05836, Republic of Korea.

出版信息

Food Chem. 2021 Oct 30;360:130063. doi: 10.1016/j.foodchem.2021.130063. Epub 2021 May 12.

Abstract

Here, we detail the biomineralization-assisted separation and concentration of crude food extract and an evaluation of its effectiveness. Schisandra chinensis fruit extract was used as a model plant extract. Hybrid grape-like mineral was assembled by calcium carbonate mineralization. The hybrid particles of S. chinensis mineral were fully characterized using field emission scanning electron microscopy, X-ray diffraction, thermogravimetric analysis, and particle size analysis. Data including the Brunauer-Emmett-Teller surface area, single point total pore volume, and adsorption/desorption analysis of pore size were also investigated. Organic molecules, including lipids such as palmitic acid, stearic acid, and linolenic acid in the Schisandra chinensis fruit, affect the formation of complex structures involving the CaCO mineralization pathway by inhibiting crystallization. However, the cosmetic active primary components were entrapped in a similar proportion in the preserved extract, and were efficiently separated without additional filtering and concentration steps for purification. In addition, the hybrid mineral was enriched (10.5 times) in Gomisin N, a representative component of S. chinensis fruit, relative to its concentration in the initial extract samples. The hybrid mineral inhibited both intracellular and extracellular melanin production and increased the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity. The data provide the first evidence of the potential use of fruit extract for obtaining hybrid minerals and the effectiveness of the biomineralization-based separation and concentration strategy.

摘要

在这里,我们详细介绍了生物矿化辅助的粗提物分离和浓缩,并评估了其效果。我们以五味子果实提取物作为模型植物提取物。通过碳酸钙矿化作用组装出杂交葡萄状矿物质。使用场发射扫描电子显微镜、X 射线衍射、热重分析和粒度分析对五味子矿化的杂交颗粒进行了全面表征。还研究了包括比表面积、单点总孔体积和孔径吸附/解吸分析在内的数据。五味子果实中的有机分子,包括棕榈酸、硬脂酸和亚油酸等脂质,通过抑制结晶,影响涉及 CaCO 矿化途径的复杂结构的形成。然而,化妆品的有效主要成分以相似的比例被包裹在保存的提取物中,无需额外的过滤和浓缩步骤即可进行纯化。此外,与初始提取物样品相比,混合矿物中五味子果实的代表性成分戈米辛 N 的浓度富集了(10.5 倍)。混合矿物抑制了细胞内和细胞外黑色素的产生,并增加了 2,2-二苯基-1-苦基肼基自由基清除活性。这些数据首次提供了利用果实提取物获得混合矿物以及基于生物矿化的分离和浓缩策略的有效性的证据。

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