Huang Kuo-Shiou, Tai Chia-Hsing, Cheng Ying-Huey, Lin Shu-Hui, Chen Tsung-Chi, Jan Fuh-Jyh
Department of Plant Pathology, National Chung Hsing University, Taichung, 40227, Taiwan.
Bureau of Animal and Plant Health Inspection and Quarantine, Council of Agriculture, Executive Yuan, Zhongzheng Dist., Taipei, 10070, Taiwan.
Arch Virol. 2017 Jul;162(7):2109-2113. doi: 10.1007/s00705-017-3260-1. Epub 2017 Mar 4.
Pepper chlorotic spot virus (PCSV), newly found in Taiwan, was identified as a new tospovirus based on the molecular characterization of its S RNA. In this study, the complete M and L RNA sequences of PCSV were determined. The M RNA has 4795 nucleotides (nts), encoding the NSm protein of 311 aa (34.5 kDa) in the viral (v) strand and the glycoprotein precursor (Gn/Gc) of 1122 aa (127.6 kDa) in the viral complementary (vc) strand. The L RNA has 8859 nts, encoding the RNA-dependent RNA polymerase (RdRp) of 2873 aa (330.8 kDa) in the vc strand. Analyses of the NSm, Gn/Gc and RdRp of PCSV revealed that PCSV is phylogenetically clustered within the watermelon silver mottle virus-related clade. Based on the whole genome sequence, PCSV is closely related to Tomato necrotic ringspot virus and should be classified as a new tospovirus species.
辣椒褪绿斑病毒（PCSV）是在台湾新发现的一种病毒，根据其S RNA的分子特征被鉴定为一种新的番茄斑萎病毒属病毒。在本研究中，测定了PCSV完整的M和L RNA序列。M RNA有4795个核苷酸（nts），在病毒（v）链上编码311个氨基酸（aa）（34.5 kDa）的NSm蛋白，在病毒互补（vc）链上编码1122个氨基酸（127.6 kDa）的糖蛋白前体（Gn/Gc）。L RNA有8859个nts，在vc链上编码2873个氨基酸（330.8 kDa）的RNA依赖的RNA聚合酶（RdRp）。对PCSV的NSm、Gn/Gc和RdRp的分析表明，PCSV在系统发育上聚集在西瓜银斑驳病毒相关的进化枝内。基于全基因组序列，PCSV与番茄坏死环斑病毒密切相关，应被归类为一种新的番茄斑萎病毒属病毒种。
