Knierim D, Blawid R, Maiss E
Faculty of Natural Sciences, Institute of Plant Diseases and Plant Protection, University of Hannover, Hannover, Germany.
Arch Virol. 2006 Sep;151(9):1761-82. doi: 10.1007/s00705-006-0749-4. Epub 2006 Apr 10.
The complete nucleotide sequence of a tospovirus isolated from Lycopersicum esculentum in Thailand was determined. The L RNA comprises of 8912 nt and codes for the RNA-dependent RNA-polymerase (RdRp) (2877 aa). Two ORFs are located on the M RNA (4823 nt) encoding the non-structural (NSm) protein (308 aa) and the viral glycoprotein precursors (Gn/Gc) (1121 aa) separated by an intergenic region of 433 nt. ORFs coding for the non-structural (NSs) and nucleocapsid (N) protein, 439 aa and 275 aa, respectively, were identified on the S RNA (3477 nt) separated by an intergenic region of 1202 nt. The N protein of the Thailand isolate was most closely related to that of capsicum chlorosis virus (CaCV), sharing an amino acid sequence identity of 92.7%. Additionally, multiple sequence analyses revealed significant similarities to tospoviruses of the species Watermelon silver mottle virus and to several putative tospovirus entries in GenBank. Based on these alignments it is proposed to refer to all these different viruses as isolates of CaCV.
测定了从泰国番茄中分离出的一种番茄斑萎病毒的完整核苷酸序列。L RNA由8912个核苷酸组成，编码依赖RNA的RNA聚合酶（RdRp）（2877个氨基酸）。M RNA（4823个核苷酸）上有两个开放阅读框，分别编码非结构蛋白（NSm）（308个氨基酸）和病毒糖蛋白前体（Gn/Gc）（1121个氨基酸），它们被一个433个核苷酸的基因间隔区隔开。在S RNA（3477个核苷酸）上鉴定出分别编码非结构蛋白（NSs）和核衣壳蛋白（N）的开放阅读框，长度分别为439个氨基酸和275个氨基酸，它们被一个1202个核苷酸的基因间隔区隔开。泰国分离株的N蛋白与辣椒褪绿病毒（CaCV）的N蛋白关系最为密切，氨基酸序列同一性为92.7%。此外，多序列分析显示，该病毒与西瓜银斑驳病毒属的番茄斑萎病毒以及GenBank中几个假定的番茄斑萎病毒条目有显著相似性。基于这些比对结果，建议将所有这些不同的病毒都称为CaCV的分离株。
