Santos H, Turner D L
Centro de Quimica Estrutural, UNL, Lisboa, Portugal.
FEBS Lett. 1987 Dec 21;226(1):179-85. doi: 10.1016/0014-5793(87)80575-6.
1H NMR saturation transfer and nuclear Overhauser effect (NOE) measurements have been used together with two-dimensional spectra to complete the assignment of the well resolved hyperfine shifted resonances in the spectrum of horse ferricytochrome c and obtain their shifts in the reduced protein. New assignments include the beta-CH2 protons of Met-80, both ring protons of His-18, and the alpha-CH2 of Gly-29 and delta-CH2 of Pro-30, which resonate surprisingly far upfield despite the absence of any Fermi contact contribution to the shift.
1H NMR饱和转移和核Overhauser效应（NOE）测量已与二维光谱一起用于完成马铁细胞色素c光谱中分辨率良好的超精细位移共振的归属，并获得它们在还原蛋白中的位移。新的归属包括Met-80的β-CH2质子、His-18的两个环质子、Gly-29的α-CH2和Pro-30的δ-CH2，尽管没有任何费米接触对位移有贡献，但它们的共振却出人意料地处于高场。
