利用核Overhauser效应进行马和金枪鱼细胞色素c铁形式的质子超精细共振归属
Proton hyperfine resonance assignments using the nuclear Overhauser effect for ferric forms of horse and tuna cytochrome c.
作者信息
Satterlee J D, Moench S
出版信息
Biophys J. 1987 Jul;52(1):101-7. doi: 10.1016/S0006-3495(87)83193-4.
Abstract
Proton hyperfine resonance assignments for cytochromes c from several species are currently being successfully pursued by several laboratories. These efforts focus mostly on the ferrous forms. In contrast to that work, we have pursued assignments of the proton hyperfine shifted resonances for horse and tuna ferricytochromes c. Our results indicate that assignments are nearly identical in those two proteins. Using the pre-steady state nuclear Overhauser effect, several additional assignments have been made for the tuna protein, whereas for the horse protein, the following protons have been assigned: heme 7, alpha CH2; heme 7, beta CH2; histidine 18, beta CH2 and alpha CH; and the methionine 80, beta CH2.
摘要
目前，几个实验室正在成功地对来自多个物种的细胞色素c进行质子超精细共振归属。这些工作主要集中在亚铁形式上。与该工作不同的是，我们对马和金枪鱼高铁细胞色素c的质子超精细位移共振进行了归属。我们的结果表明，这两种蛋白质的归属几乎相同。利用预稳态核Overhauser效应，对金枪鱼蛋白质进行了一些额外的归属，而对于马蛋白质，已归属的质子如下：血红素7的α-CH₂；血红素7的β-CH₂；组氨酸18的β-CH₂和α-CH；以及甲硫氨酸80的β-CH₂。
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