基于纳米技术平台的寨卡病毒感染诊断。
Diagnosis of Zika virus infection on a nanotechnology platform.
机构信息
Nirmidas Biotech, Palo Alto, California, USA.
Department of Pathology, Stanford University School of Medicine, Stanford, California, USA.
出版信息
Nat Med. 2017 May;23(5):548-550. doi: 10.1038/nm.4302. Epub 2017 Mar 6.
We developed a multiplexed assay on a plasmonic-gold platform for measuring IgG and IgA antibodies and IgG avidity against both Zika virus (ZIKV) and dengue virus (DENV) infections. In contrast to IgM cross-reactivity, IgG and IgA antibodies against ZIKV nonstructural protein 1 (NS1) antigen were specific to ZIKV infection, and IgG avidity revealed recent ZIKV infection and past DENV-2 infection in patients in dengue-endemic regions. This assay could enable specific diagnosis of ZIKV infection over other flaviviral infections.
我们开发了一种基于等离子金平台的多重分析检测方法，用于测量针对寨卡病毒（ZIKV）和登革热病毒（DENV）感染的 IgG 和 IgA 抗体以及 IgG 亲和力。与 IgM 交叉反应不同，针对寨卡病毒非结构蛋白 1（NS1）抗原的 IgG 和 IgA 抗体特异性针对寨卡病毒感染，而 IgG 亲和力则揭示了登革热流行地区患者近期的寨卡病毒感染和过去的登革热 2 型感染。该检测方法可以特异性诊断寨卡病毒感染，而不会与其他黄病毒感染混淆。
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