• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

鉴定具有血清学检测应用潜力的寨卡病毒 NS1 衍生肽。

Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests.

机构信息

Laboratório de Imunobiotecnologia, Centro de Imunologia, Instituto Adolfo Lutz, Av. Dr. Arnaldo, 351, São Paulo 01246-902, SP, Brazil.

Instituto de Biociências Letras e Ciências Exatas, Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Cristóvão Colombo, 2265, São Jose do Rio Preto 15054-000, SP, Brazil.

出版信息

Viruses. 2023 Feb 28;15(3):654. doi: 10.3390/v15030654.

DOI:10.3390/v15030654
PMID:36992364
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10052002/
Abstract

Zika virus (ZIKV), a mosquito-borne pathogen, is an emerging arbovirus associated with sporadic symptomatic cases of great medical concern, particularly among pregnant women and newborns affected with neurological disorders. Serological diagnosis of ZIKV infection is still an unmet challenge due to the co-circulation of the dengue virus, which shares extensive sequence conservation of structural proteins leading to the generation of cross-reactive antibodies. In this study, we aimed to obtain tools for the development of improved serological tests for the detection of ZIKV infection. Polyclonal sera (pAb) and a monoclonal antibody (mAb 2F2) against a recombinant form of the ZIKV nonstructural protein 1 (NS1) allowed the identification of linear peptide epitopes of the NS1 protein. Based on these findings, six chemically synthesized peptides were tested both in dot blot and ELISA assays using convalescent sera collected from ZIKV-infected patients. Two of these peptides specifically detected the presence of ZIKV antibodies and proved to be candidates for the detection of ZIKV-infected subjects. The availability of these tools opens perspectives for the development of NS1-based serological tests with enhanced sensitivity regarding other flaviviruses.

摘要

Zika 病毒（ZIKV）是一种蚊媒病原体，属于新兴的虫媒病毒，与散发性有症状病例密切相关，尤其是在受神经系统疾病影响的孕妇和新生儿中。由于登革热病毒的共同传播，ZIKV 感染的血清学诊断仍然是一个未满足的挑战，因为这两种病毒的结构蛋白具有广泛的序列保守性，导致产生交叉反应性抗体。在这项研究中，我们旨在获得用于开发改进的血清学检测方法的工具，以检测 ZIKV 感染。针对 Zika 病毒非结构蛋白 1（NS1）的重组形式的多克隆血清（pAb）和单克隆抗体（mAb 2F2）允许鉴定 NS1 蛋白的线性肽表位。基于这些发现，使用从 ZIKV 感染患者中收集的恢复期血清，在斑点印迹和 ELISA 检测中测试了六个化学合成的肽。其中两个肽特异性检测到 ZIKV 抗体的存在，被证明是检测 ZIKV 感染患者的候选物。这些工具的可用性为基于 NS1 的血清学检测方法的开发开辟了前景，这些方法在针对其他黄病毒方面具有更高的敏感性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b7f/10052002/a617f49022a2/viruses-15-00654-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b7f/10052002/5d6e768f36ec/viruses-15-00654-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b7f/10052002/be68c0afb416/viruses-15-00654-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b7f/10052002/b1580303ca88/viruses-15-00654-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b7f/10052002/700c53941cde/viruses-15-00654-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b7f/10052002/62c9d9b77ca6/viruses-15-00654-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b7f/10052002/a617f49022a2/viruses-15-00654-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b7f/10052002/5d6e768f36ec/viruses-15-00654-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b7f/10052002/be68c0afb416/viruses-15-00654-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b7f/10052002/b1580303ca88/viruses-15-00654-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b7f/10052002/700c53941cde/viruses-15-00654-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b7f/10052002/62c9d9b77ca6/viruses-15-00654-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b7f/10052002/a617f49022a2/viruses-15-00654-g006.jpg

相似文献

1
Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests.鉴定具有血清学检测应用潜力的寨卡病毒 NS1 衍生肽。
Viruses. 2023 Feb 28;15(3):654. doi: 10.3390/v15030654.
2
ZIKV-Specific NS1 Epitopes as Serological Markers of Acute Zika Virus Infection.寨卡病毒特异性 NS1 表位作为急性寨卡病毒感染的血清学标志物。
J Infect Dis. 2019 Jun 19;220(2):203-212. doi: 10.1093/infdis/jiz092.
3
Non-structural protein 1 from Zika virus: Heterologous expression, purification, and potential for diagnosis of Zika infections.寨卡病毒非结构蛋白1：异源表达、纯化及用于寨卡病毒感染诊断的潜力
Int J Biol Macromol. 2021 Sep 1;186:984-993. doi: 10.1016/j.ijbiomac.2021.07.083. Epub 2021 Jul 19.
4
Serodiagnosis of Zika virus (ZIKV) infections by a novel NS1-based ELISA devoid of cross-reactivity with dengue virus antibodies: a multicohort study of assay performance, 2015 to 2016.基于新型NS1的ELISA法对寨卡病毒（ZIKV）感染进行血清学诊断，该方法与登革热病毒抗体无交叉反应：2015至2016年检测性能的多队列研究
Euro Surveill. 2016 Dec 15;21(50). doi: 10.2807/1560-7917.ES.2016.21.50.30426.
5
Comprehensive Evaluation of Differential Serodiagnosis between Zika and Dengue Viral Infections. Zika 和登革热病毒感染的血清学诊断差异的综合评估。
J Clin Microbiol. 2019 Feb 27;57(3). doi: 10.1128/JCM.01506-18. Print 2019 Mar.
6
Monoclonal Antibodies against Zika Virus NS1 Protein Confer Protection via Fc Receptor-Dependent and -Independent Pathways.抗寨卡病毒 NS1 蛋白的单克隆抗体通过 Fc 受体依赖性和非依赖性途径发挥保护作用。
mBio. 2021 Feb 9;12(1):e03179-20. doi: 10.1128/mBio.03179-20.
7
Engineered NS1 for Sensitive, Specific Zika Virus Diagnosis from Patient Serology.工程化 NS1 用于从患者血清学中进行敏感、特异的寨卡病毒诊断。
Emerg Infect Dis. 2021 May;27(5):1427-1437. doi: 10.3201/eid2705.190121.
8
Delayed and highly specific antibody response to nonstructural protein 1 (NS1) revealed during natural human ZIKV infection by NS1-based capture ELISA.通过基于 NS1 的捕获 ELISA 发现，在自然发生的人类 ZIKV 感染期间，针对非结构蛋白 1（NS1）的延迟和高度特异性抗体反应。
BMC Infect Dis. 2018 Jun 14;18(1):275. doi: 10.1186/s12879-018-3173-y.
9
Zika Virus Non-Structural Protein 1 Antigen-Capture Immunoassay. Zika 病毒非结构蛋白 1 抗原捕获免疫测定法。
Viruses. 2021 Sep 5;13(9):1771. doi: 10.3390/v13091771.
10
Combination of Nonstructural Protein 1-Based Enzyme-Linked Immunosorbent Assays Can Detect and Distinguish Various Dengue Virus and Zika Virus Infections.基于非结构蛋白 1 的酶联免疫吸附测定的联合应用可检测和区分不同的登革热病毒和 Zika 病毒感染。
J Clin Microbiol. 2019 Jan 30;57(2). doi: 10.1128/JCM.01464-18. Print 2019 Feb.

引用本文的文献

1
Production of Recombinant Zika Virus Envelope Protein by Airlift Bioreactor as a New Subunit Vaccine Platform.气升式生物反应器生产重组寨卡病毒包膜蛋白作为新型亚单位疫苗平台。
Int J Mol Sci. 2023 Sep 11;24(18):13955. doi: 10.3390/ijms241813955.

本文引用的文献

1
Surveillance of Zika and Dengue viruses in field-collected Aedes aegypti mosquitoes from different states of India.对来自印度不同邦的野外采集的埃及伊蚊中寨卡病毒和登革热病毒的监测。
Virology. 2022 Sep;574:96-101. doi: 10.1016/j.virol.2022.07.004. Epub 2022 Aug 6.
2
Susceptibility to endemic Aedes-borne viruses among pregnant women in Risaralda, Colombia.哥伦比亚里萨拉尔达省孕妇对地方性伊蚊传播病毒的易感性。
Int J Infect Dis. 2022 Sep;122:832-840. doi: 10.1016/j.ijid.2022.07.017. Epub 2022 Jul 9.
3
Combined detection of molecular and serological signatures of viral infections: The dual assay concept.
联合检测病毒感染的分子和血清学特征：双检测概念。
Biosens Bioelectron. 2022 Aug 15;210:114302. doi: 10.1016/j.bios.2022.114302. Epub 2022 Apr 25.
4
Detection of Zika virus disease in Thiruvananthapuram, Kerala, India 2021 during the second wave of COVID-19 pandemic.2021年在印度喀拉拉邦特里凡得琅第二波新冠疫情期间寨卡病毒病的检测情况
J Med Virol. 2022 Jun;94(6):2346-2349. doi: 10.1002/jmv.27638. Epub 2022 Feb 8.
5
Zika Virus Non-Structural Protein 1 Antigen-Capture Immunoassay. Zika 病毒非结构蛋白 1 抗原捕获免疫测定法。
Viruses. 2021 Sep 5;13(9):1771. doi: 10.3390/v13091771.
6
NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus.基于NS1的酶联免疫吸附测定试验能有效检测登革热感染，且与寨卡病毒无交叉反应。
Int J Infect Dis. 2021 Nov;112:202-204. doi: 10.1016/j.ijid.2021.09.009. Epub 2021 Sep 20.
7
Linear and Continuous Flavivirus Epitopes From Naturally Infected Humans.线性和连续黄病毒表位来自自然感染的人类。
Front Cell Infect Microbiol. 2021 Aug 12;11:710551. doi: 10.3389/fcimb.2021.710551. eCollection 2021.
8
A broadly protective antibody that targets the flavivirus NS1 protein.一种广泛保护性的抗体，针对黄病毒 NS1 蛋白。
Science. 2021 Jan 8;371(6525):190-194. doi: 10.1126/science.abb9425.
9
Zika virus serological diagnosis: commercial tests and monoclonal antibodies as tools.寨卡病毒血清学诊断：作为工具的商业检测与单克隆抗体
J Venom Anim Toxins Incl Trop Dis. 2020 Nov 18;26:e20200019. doi: 10.1590/1678-9199-JVATITD-2020-0019.
10
The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research.ARRIVE 指南 2.0：报告动物研究的更新指南。
PLoS Biol. 2020 Jul 14;18(7):e3000410. doi: 10.1371/journal.pbio.3000410. eCollection 2020 Jul.