Lan C Y, Igo M M
Division of Biological Sciences, University of California, Davis, 95616, USA.
J Bacteriol. 1998 Jan;180(1):171-4. doi: 10.1128/JB.180.1.171-174.1998.
We have generated a mutant form of the OmpR regulatory protein, OmpRD55E, that is active independent of the EnvZ kinase. Notably, the pattern of OmpF and OmpC expression can be altered simply by changing the level of this mutant protein in the cell. This result supports a key prediction of the current model of porin regulation, which states that the differential regulation of OmpF and OmpC is a direct consequence of the cellular level of the active form of OmpR.
我们已经生成了一种OmpR调节蛋白的突变形式OmpRD55E,它的活性不依赖于EnvZ激酶。值得注意的是,仅通过改变细胞中这种突变蛋白的水平,就可以改变OmpF和OmpC的表达模式。这一结果支持了当前孔蛋白调节模型的一个关键预测,该模型指出,OmpF和OmpC的差异调节是OmpR活性形式细胞水平的直接结果。