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Differential expression of the OmpF and OmpC porin proteins in Escherichia coli K-12 depends upon the level of active OmpR.大肠杆菌K-12中OmpF和OmpC孔蛋白的差异表达取决于活性OmpR的水平。
J Bacteriol. 1998 Jan;180(1):171-4. doi: 10.1128/JB.180.1.171-174.1998.
2
A phosphorylation site mutant of OmpR reveals different binding conformations at ompF and ompC.OmpR的磷酸化位点突变体在ompF和ompC处显示出不同的结合构象。
J Mol Biol. 2002 Jan 25;315(4):497-511. doi: 10.1006/jmbi.2001.5222.
3
Relative binding affinities of OmpR and OmpR-phosphate at the ompF and ompC regulatory sites.OmpR和磷酸化OmpR在ompF和ompC调控位点的相对结合亲和力。
J Mol Biol. 1998 Sep 4;281(5):857-70. doi: 10.1006/jmbi.1998.1985.
4
EnvZ-independent phosphotransfer signaling pathway of the OmpR-mediated osmoregulatory expression of OmpC and OmpF in Escherichia coli.大肠杆菌中OmpR介导的OmpC和OmpF渗透调节表达的不依赖EnvZ的磷酸转移信号通路。
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EnvZ functions through OmpR to control porin gene expression in Escherichia coli K-12.EnvZ通过OmpR发挥作用,以控制大肠杆菌K-12中孔蛋白基因的表达。
J Bacteriol. 1988 Jan;170(1):439-41. doi: 10.1128/jb.170.1.439-441.1988.

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本文引用的文献

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Signal transduction via the histidyl-aspartyl phosphorelay.通过组氨酰-天冬氨酰磷酰基转移进行的信号转导。
Genes Cells. 1997 Mar;2(3):167-84. doi: 10.1046/j.1365-2443.1997.d01-311.x.
2
Function of conserved histidine-243 in phosphatase activity of EnvZ, the sensor for porin osmoregulation in Escherichia coli.保守组氨酸-243在EnvZ磷酸酶活性中的作用,EnvZ是大肠杆菌中孔蛋白渗透调节的传感器。
J Bacteriol. 1997 Jun;179(11):3729-35. doi: 10.1128/jb.179.11.3729-3735.1997.
3
Role of His243 in the phosphatase activity of EnvZ in Escherichia coli.组氨酸243在大肠杆菌EnvZ磷酸酶活性中的作用。
J Bacteriol. 1997 Feb;179(4):1413-6. doi: 10.1128/jb.179.4.1413-1416.1997.
4
Altering the level and regulation of the major sigma subunit of RNA polymerase affects gene expression and development in Bacillus subtilis.改变RNA聚合酶主要σ亚基的水平和调控会影响枯草芽孢杆菌中的基因表达和发育。
Mol Microbiol. 1996 Apr;20(1):201-12. doi: 10.1111/j.1365-2958.1996.tb02501.x.
5
Glutamate at the site of phosphorylation of nitrogen-regulatory protein NTRC mimics aspartyl-phosphate and activates the protein.氮调节蛋白NTRC磷酸化位点处的谷氨酸模拟天冬氨酰磷酸并激活该蛋白。
J Mol Biol. 1993 Jul 5;232(1):67-78. doi: 10.1006/jmbi.1993.1370.
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The essential tension: opposed reactions in bacterial two-component regulatory systems.本质张力:细菌双组分调节系统中的相反反应
Trends Microbiol. 1993 Nov;1(8):306-10. doi: 10.1016/0966-842x(93)90007-e.
7
Cloning of the regulatory genes (ompR and envZ) for the matrix proteins of the Escherichia coli outer membrane.大肠杆菌外膜基质蛋白调控基因(ompR和envZ)的克隆
J Bacteriol. 1982 Jun;150(3):1462-6. doi: 10.1128/jb.150.3.1462-1466.1982.
8
The ompB locus and the regulation of the major outer membrane porin proteins of Escherichia coli K12.大肠杆菌K12的ompB基因座与主要外膜孔蛋白的调控
J Mol Biol. 1981 Feb 15;146(1):23-43. doi: 10.1016/0022-2836(81)90364-8.
9
The tolC locus of Escherichia coli affects the expression of three major outer membrane proteins.大肠杆菌的tolC基因座影响三种主要外膜蛋白的表达。
J Bacteriol. 1982 Jun;150(3):1016-23. doi: 10.1128/jb.150.3.1016-1023.1982.
10
Mutations in a new chromosomal gene of Escherichia coli K-12, pcnB, reduce plasmid copy number of pBR322 and its derivatives.大肠杆菌K-12的一个新染色体基因pcnB发生突变,会降低pBR322及其衍生物的质粒拷贝数。
Mol Gen Genet. 1986 Nov;205(2):285-90. doi: 10.1007/BF00430440.

大肠杆菌K-12中OmpF和OmpC孔蛋白的差异表达取决于活性OmpR的水平。

Differential expression of the OmpF and OmpC porin proteins in Escherichia coli K-12 depends upon the level of active OmpR.

作者信息

Lan C Y, Igo M M

机构信息

Division of Biological Sciences, University of California, Davis, 95616, USA.

出版信息

J Bacteriol. 1998 Jan;180(1):171-4. doi: 10.1128/JB.180.1.171-174.1998.

DOI:10.1128/JB.180.1.171-174.1998
PMID:9422609
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC106865/
Abstract

We have generated a mutant form of the OmpR regulatory protein, OmpRD55E, that is active independent of the EnvZ kinase. Notably, the pattern of OmpF and OmpC expression can be altered simply by changing the level of this mutant protein in the cell. This result supports a key prediction of the current model of porin regulation, which states that the differential regulation of OmpF and OmpC is a direct consequence of the cellular level of the active form of OmpR.

摘要

我们已经生成了一种OmpR调节蛋白的突变形式OmpRD55E,它的活性不依赖于EnvZ激酶。值得注意的是,仅通过改变细胞中这种突变蛋白的水平,就可以改变OmpF和OmpC的表达模式。这一结果支持了当前孔蛋白调节模型的一个关键预测,该模型指出,OmpF和OmpC的差异调节是OmpR活性形式细胞水平的直接结果。