Chevrier D, Vézina C, Bastille J, Linard C, Sonenberg N, Boileau G
Département de Biochimie, Faculté de Médecine, Université de Montréal, Québec, Canada.
J Biol Chem. 1988 Jan 15;263(2):902-10.
The SP6 polymerase/promoter system was used to synthesize porcine pro-opiomelanocortin mRNAs with nucleotide sequence deletions in the 5'- as well as 3'-untranslated and coding regions. The translational efficiency of the mutant mRNAs was evaluated by cell-free translation or by monitoring the rate and extent of ribosome binding in the presence of sparsomycin. The results of these experiments indicate that specific nucleotide sequences in the 5'-untranslated and coding regions of the pro-opiomelanocortin mRNA decrease its rate of translation. Structure mapping of the mRNA with double-strand and single-strand specific nucleases suggests that these sequences can form stable secondary structures.
利用SP6聚合酶/启动子系统合成了猪阿黑皮素原mRNA，其5'-非翻译区、3'-非翻译区及编码区的核苷酸序列均有缺失。通过无细胞翻译或监测在稀疏霉素存在下核糖体结合的速率和程度来评估突变mRNA的翻译效率。这些实验结果表明，阿黑皮素原mRNA的5'-非翻译区和编码区中的特定核苷酸序列降低了其翻译速率。用双链和单链特异性核酸酶对mRNA进行结构图谱分析表明，这些序列可形成稳定的二级结构。
