Fütterer J, Gordon K, Bonneville J M, Sanfaçon H, Pisan B, Penswick J, Hohn T
Friedrich Miescher-Institut, Basel, Switzerland.
Nucleic Acids Res. 1988 Sep 12;16(17):8377-90. doi: 10.1093/nar/16.17.8377.
The 600 nt long sequences preceeding the first large ORFs (ORF VII) of three caulimoviruses, although varying in primary sequence, can be folded into a large stem/loop structure centered around a conserved stretch of 36 nucleotides. Deletions of the conserved sequence delay symptom appearance considerably, but do not affect expression of a reporter gene in plant protoplasts. Another striking similarity between the leaders concerns the number and distribution of small open reading frames (sORF) they carry. Expression of two of these sORFs was tested by fusion of a reporter gene: both were expressed in plant protoplasts.
三种花椰菜花叶病毒的第一个大开放阅读框（ORF VII）之前的600个核苷酸长的序列，尽管一级序列不同，但可折叠成一个以36个核苷酸的保守序列为中心的大茎环结构。保守序列的缺失会显著延迟症状出现，但不影响植物原生质体中报告基因的表达。这些前导序列之间的另一个显著相似之处在于它们所携带的小开放阅读框（sORF）的数量和分布。通过报告基因融合测试了其中两个sORF的表达：两者都在植物原生质体中表达。
