接触农药的男性农民体内的农药代谢物与氧化应激
Pesticide metabolite and oxidative stress in male farmers exposed to pesticide.
作者信息
Lee Kang Myoung, Park Sang-Yoo, Lee Kyungsuk, Oh Sung-Soo, Ko Sang Baek
机构信息
Department of Medicine, The Graduate School, Yonsei University, Seoul, South Korea.
Department of Otolaryngology Head and Neck Surgery, Wonju College of Medicine, Yonsei Unversity, Seoul, South Korea.
出版信息
Ann Occup Environ Med. 2017 Feb 28;29:5. doi: 10.1186/s40557-017-0162-3. eCollection 2017.
BACKGROUND
The objective of this study was to measure malondialdehyde (MDA) and isoprostane which has been used as an index of lipid injury, 8-hydroxy-2'-deoxyguanosine (8-OHdG), which has been used as an index of DNA damage, and dialkyl-phosphate (DAP), which has been used to quantify pesticide exposure, and to investigate the relationship between pesticide exposure and oxidative stress.
METHODS
This study was a cross-sectional study that evaluated 84 male farmers exposure to pesticide. In this study, 8-OHdG, isoprostane, and MDA were measured as oxidative stress indices, and dialkyl-phosphate (dimethylphosphate(DMP), diethylphosphate(DEP), dimethylthiophosphate(DMTP), and diethylthiophosphate (DETP)) excreted in the urine was also measured to evaluate pesticide exposure. A linear regression analysis was performed to investigate the relationship between pesticide metabolites, and oxidative stress biomarkers.
RESULTS
A Correlation analysis was performed for pesticide exposure month (PEI), cumulative exposure index (CEI), and DAP as well as the concentration of the oxidative stress biomarkers. The PEM significantly and positively correlated to the levels of 8-OHdG, isoprostane, CEI, and DMP. CEI showed a correlation to 8-OHdG and PEM. DMP, DEP, and DETP showed a positive correlation to 8-OHdG, isoprostane, and MDA. A correlation analysis was adjusted some demographic characteristics, such as age, smoking, drinking, and exercise to determine the relationship between pesticide exposure and oxidative stress. The 8-OHdG, isoprostane, and MDA levels were significantly related to the DMP (ß = 0.320), DEP (ß = 0.390), and DETP (ß = 0.082); DMP (ß = 0.396), DEP (ß = 0.508), and DETP (ß = 0.504); and DMP (ß = 0.432), DEP (ß = 0.508), and DETP (ß = 0.329) levels, respectively.
CONCLUSIONS
The concentration between oxidative stress biomarkers and the pesticide metabolite were a positive correlation. Indicators of oxidative stress was associated with a pesticide metabolite DMP, DEP, and DETP. Therefore, Pesticide exposure and oxidative stress were relevant.
背景
本研究的目的是测量已被用作脂质损伤指标的丙二醛（MDA）和异前列腺素、已被用作DNA损伤指标的8-羟基-2'-脱氧鸟苷（8-OHdG）以及已被用于量化农药暴露的二烷基磷酸酯（DAP），并研究农药暴露与氧化应激之间的关系。
方法
本研究为横断面研究，评估了84名接触农药的男性农民。在本研究中，测量了8-OHdG、异前列腺素和MDA作为氧化应激指标，还测量了尿液中排泄的二烷基磷酸酯（二甲基磷酸酯（DMP）、二乙基磷酸酯（DEP）、二甲基硫代磷酸酯（DMTP）和二乙基硫代磷酸酯（DETP））以评估农药暴露。进行线性回归分析以研究农药代谢物与氧化应激生物标志物之间的关系。
结果
对农药暴露月（PEI）、累积暴露指数（CEI）、DAP以及氧化应激生物标志物的浓度进行了相关性分析。PEI与8-OHdG、异前列腺素、CEI和DMP的水平显著正相关。CEI与8-OHdG和PEM相关。DMP、DEP和DETP与8-OHdG、异前列腺素和MDA呈正相关。调整了一些人口统计学特征，如年龄、吸烟、饮酒和运动后进行相关性分析，以确定农药暴露与氧化应激之间的关系。8-OHdG、异前列腺素和MDA水平分别与DMP（β = 0.320）、DEP（β = 0.390）和DETP（β = 0.082）；DMP（β = 0.396）、DEP（β = 0.508）和DETP（β = 0.504）；以及DMP（β = 0.432）、DEP（β = 0.508）和DETP（β = 0.329）水平显著相关。
结论
氧化应激生物标志物与农药代谢物之间的浓度呈正相关。氧化应激指标与农药代谢物DMP、DEP和DETP相关。因此，农药暴露与氧化应激相关。
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