利用聚合酶链反应检测石蜡包埋组织中的人乳头瘤病毒。
Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction.
作者信息
Shibata D K, Arnheim N, Martin W J
机构信息
Department of Pathology, Los Angeles County-University of Southern California Medical Center 90033.
出版信息
J Exp Med. 1988 Jan 1;167(1):225-30. doi: 10.1084/jem.167.1.225.
Abstract
Human papilloma virus (HPV) DNA sequences have been detected in paraffin-embedded tissue using an enzymatic in vitro amplification technique known as the polymerase chain reaction. Amplification of a HPV DNA sequence before its detection with a cDNA probe significantly increases the rapidity as well as the sensitivity of detection such that a single 5-10-micron thick paraffin-embedded tissue section can be analyzed within 24 h. The assay specifically detected HPV 16 or 18 without crossreactivity with HPV 6 or 11. As few as 20 viral copies could be detected. The rapid and sensitive analysis of HPV in normal and pathological tissues using this technique may contribute significantly to identifying the role of HPV as a risk factor in carcinoma.
摘要
利用一种称为聚合酶链反应的体外酶促扩增技术，已在石蜡包埋组织中检测到人乳头瘤病毒（HPV）的DNA序列。在用cDNA探针检测之前对HPV DNA序列进行扩增，可显著提高检测的速度和灵敏度，从而能在24小时内分析单个5 - 10微米厚的石蜡包埋组织切片。该检测方法能特异性检测HPV 16或18，与HPV 6或11无交叉反应。低至20个病毒拷贝都能被检测到。使用该技术对正常组织和病理组织中的HPV进行快速灵敏分析，可能对确定HPV作为癌症风险因素的作用有重大贡献。
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