Schneider-Gädicke A, Schwarz E
EMBO J. 1986 Sep;5(9):2285-92. doi: 10.1002/j.1460-2075.1986.tb04496.x.
Transcription of human papillomavirus type 18 (HPV18) DNA in the human cervical carcinoma cell lines HeLa, C4-1 and SW756 was studied by nucleotide sequence analysis of HPV18-positive cDNA clones isolated from a HeLa, C4-1 and SW756 cDNA library, respectively, and the cDNA sequences were used to predict the potential encoded proteins. The cDNA clones from all three cell lines were found to be derived from virus-cell fusion transcripts in which 3'-terminal host cell sequences (different for each cell line) were spliced to 5'-terminal exon sequences from the HPV18 E6-E7-E1 region. Three different types of cDNA clones can be distinguished according to the splicing patterns observed in the 5' terminal HPV18 sequences. They carry as potential protein-coding regions the HPV18 specific open reading frames E6 and E6* (generated by splicing and identical with E6 up to the E6* splice junction), E7 and E1 (only in HeLa). Translation of specific cellular genes from the chimeric viral-cellular transcripts seems to be unlikely. The mapping of the 5'-ends of the virus-cell fusion transcripts indicates that transcription is initiated at a viral promoter. The similar patterns of HPV18 transcription in the three different cervical carcinoma cell lines suggest a functional role of HPV18 early genes for the malignant phenotype of these cells.
通过对分别从HeLa、C4 - 1和SW756 cDNA文库中分离得到的HPV18阳性cDNA克隆进行核苷酸序列分析，研究了人乳头瘤病毒18型（HPV18）DNA在人宫颈癌细胞系HeLa、C4 - 1和SW756中的转录情况，并利用这些cDNA序列预测潜在的编码蛋白。发现来自所有三个细胞系的cDNA克隆均源自病毒 - 细胞融合转录本，其中3'末端宿主细胞序列（每个细胞系不同）与HPV18 E6 - E7 - E1区域的5'末端外显子序列拼接。根据在5'末端HPV18序列中观察到的剪接模式，可以区分出三种不同类型的cDNA克隆。它们作为潜在的蛋白质编码区域携带HPV18特异性开放阅读框E6和E6*（通过剪接产生，在E6*剪接连接处之前与E6相同）、E7和E1（仅在HeLa中）。嵌合病毒 - 细胞转录本中特定细胞基因的翻译似乎不太可能。病毒 - 细胞融合转录本5'末端的定位表明转录起始于病毒启动子。三种不同宫颈癌细胞系中HPV18转录的相似模式表明HPV18早期基因对这些细胞的恶性表型具有功能性作用。
