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用于超宽带光谱域光相干断层成像的 K 空间光谱仪设计。

Design of a k-space spectrometer for ultra-broad waveband spectral domain optical coherence tomography.

机构信息

Visual and Biomedical Optics Lab, The Ohio State University, Columbus, OH 43212, USA.

Department of Ophthalmology and Visual Science, The Ohio State University, Columbus, OH 43212, USA.

出版信息

Sci Rep. 2017 Mar 7;7:42353. doi: 10.1038/srep42353.

DOI:10.1038/srep42353
PMID:28266502
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5339709/
Abstract

Nonlinear sampling of the interferograms in wavenumber (k) space degrades the depth-dependent signal sensitivity in conventional spectral domain optical coherence tomography (SD-OCT). Here we report a linear-in-wavenumber (k-space) spectrometer for an ultra-broad bandwidth (760 nm-920 nm) SD-OCT, whereby a combination of a grating and a prism serves as the dispersion group. Quantitative ray tracing is applied to optimize the linearity and minimize the optical path differences for the dispersed wavenumbers. Zemax simulation is used to fit the point spread functions to the rectangular shape of the pixels of the line-scan camera and to improve the pixel sampling rates. An experimental SD-OCT is built to test and compare the performance of the k-space spectrometer with that of a conventional one. Design results demonstrate that this k-space spectrometer can reduce the nonlinearity error in k-space from 14.86% to 0.47% (by approximately 30 times) compared to the conventional spectrometer. The 95% confidence interval for RMS diameters is 5.48 ± 1.76 μm-significantly smaller than both the pixel size (14 μm × 28 μm) and the Airy disc (25.82 μm in diameter, calculated at the wavenumber of 7.548 μm). Test results demonstrate that the fall-off curve from the k-space spectrometer exhibits much less decay (maximum as -5.20 dB) than the conventional spectrometer (maximum as -16.84 dB) over the whole imaging depth (2.2 mm).

摘要

在传统的光谱域光学相干断层扫描 (SD-OCT) 中,在波数 (k) 空间中对干涉图进行非线性采样会降低深度相关信号的灵敏度。在这里,我们报告了一种用于超宽带宽 (760nm-920nm) SD-OCT 的线性波数 (k 空间) 光谱仪,其中光栅和棱镜的组合用作色散组。定量光线追踪用于优化线性度并最小化色散波数的光程差。使用 Zemax 模拟来拟合点扩散函数到线扫描相机的像素的矩形形状,并提高像素采样率。构建了一个实验性的 SD-OCT 来测试和比较 k 空间光谱仪与传统光谱仪的性能。设计结果表明,与传统光谱仪相比,这种 k 空间光谱仪可以将 k 空间中的非线性误差从 14.86%降低到 0.47%(约 30 倍)。95%置信区间的 RMS 直径为 5.48±1.76μm,显著小于像素尺寸(14μm×28μm)和艾里斑(7.548μm 波数处的直径为 25.82μm)。测试结果表明,k 空间光谱仪的衰减曲线(最大为-5.20dB)比传统光谱仪(最大为-16.84dB)在整个成像深度(2.2mm)内的衰减要小得多。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aee8/5339709/219f0916ff6d/srep42353-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aee8/5339709/7f5f0939a455/srep42353-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aee8/5339709/21d5c63c8ef6/srep42353-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aee8/5339709/219f0916ff6d/srep42353-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aee8/5339709/7f5f0939a455/srep42353-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aee8/5339709/21d5c63c8ef6/srep42353-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aee8/5339709/219f0916ff6d/srep42353-f3.jpg

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