Tachibana Shin-Ichiro, Kawai Satoru, Katakai Yuko, Takahashi Hideo, Nakade Toru, Yasutomi Yasuhiro, Horii Toshihiro, Tanabe Kazuyuki
Laboratory of Malariology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan; Department of Biology and Geosciences, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, Japan.
Laboratory of Tropical Medicine and Parasitology, Institute of International Education and Research, Dokkyo Medical University, Shimotsuga, Tochigi 321-0293, Japan.
Parasitol Int. 2015 Jun;64(3):274-81. doi: 10.1016/j.parint.2014.10.004. Epub 2014 Oct 12.
Although the human malaria parasite Plasmodium vivax is closely related to Asian Old World monkey malaria parasites, there are no reports of P. vivax infections in macaques. In this study, we compared the infectivity of P. vivax and Plasmodium cynomolgi in Japanese macaques (Macaca fuscata) and in cynomolgus macaques (Macaca fascicularis). The Japanese macaques were highly susceptible to P. cynomolgi but not to P. vivax, whereas cynomolgus macaques showed mild/limited P. cynomolgi infection and were, also, not susceptible to P. vivax. Serotyping and amino acid sequence comparison of erythrocyte surface Duffy antigen/receptor for chemokines (DARC) indicate that the Japanese macaque DARC sequence is nearly identical to that of rhesus (Macaca mulatta) and cynomolgus macaques. This suggests that the macaques share a common mechanism for preventing P. vivax infection. Comparison of amino acid sequences of the Duffy-binding-like (DBL) domain from several different Plasmodium species suggests that P. vivax DBLs will not bind to macaque DARCs, which can explain the lack of P. vivax infectivity. The DBL sequence analyses also suggest that P. cynomolgi DBLs may target Japanese macaque erythrocytes through a DARC-independent interaction.
尽管人类疟原虫间日疟原虫与亚洲旧世界猴疟原虫密切相关，但尚无猕猴感染间日疟原虫的报道。在本研究中，我们比较了间日疟原虫和食蟹猴疟原虫对日本猕猴（猕猴）和食蟹猴（食蟹猕猴）的感染性。日本猕猴对食蟹猴疟原虫高度易感，但对间日疟原虫不易感，而食蟹猴表现出轻度/有限的食蟹猴疟原虫感染，对间日疟原虫也不易感。红细胞表面趋化因子达菲抗原/受体（DARC）的血清分型和氨基酸序列比较表明，日本猕猴的DARC序列与恒河猴（恒河猴）和食蟹猴的序列几乎相同。这表明猕猴具有预防间日疟原虫感染的共同机制。对几种不同疟原虫物种的类达菲结合（DBL）结构域氨基酸序列的比较表明，间日疟原虫的DBL不会与猕猴的DARC结合，这可以解释间日疟原虫缺乏感染性的原因。DBL序列分析还表明，食蟹猴疟原虫的DBL可能通过一种不依赖DARC的相互作用靶向日本猕猴红细胞。
