Department of Plant & Environmental New Resources, College of Life Sciences, Kyung Hee University, 1 Seocheon-dong, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea.
Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University, Republic of Korea.
Carbohydr Polym. 2017 May 1;163:34-42. doi: 10.1016/j.carbpol.2017.01.052. Epub 2017 Jan 18.
Crosslinked chitosan was prepared by Schiff base formation between the aldehyde groups of dialdehyde cellulose (DAC) and the amino groups of chitosan and a subsequent reduction. DAC was obtained through periodate oxidation of cellulose and solubilization in hot water at 100°C for 1h. Three grades of DAC-crosslinked chitosan were prepared by adding various amounts DAC. The degrees of crosslinking as determined by amino group content were 3.8, 8.3, and 12.1%, respectively. DAC-crosslinked chitosan showed higher stability in the pH 2-9 range and no cytotoxicity was identified over the course of a 21-day long-term stability test. Also, DAC-crosslinked chitosan showed remarkably high bovine serum albumin (BSA) adsorption capacity at pH 5.5 as a result of the increased amino group content, due to the reaction between DAC and chitosan molecular chains occurring at multiple points even though DAC-crosslinked chitosan showed a lower degree of crosslinking.
交联壳聚糖是通过二醛纤维素 (DAC) 的醛基与壳聚糖的氨基之间的席夫碱形成以及随后的还原反应制备的。DAC 通过纤维素的高碘酸盐氧化和在 100°C 的热水中 1h 的溶解获得。通过添加不同量的 DAC 制备了三种等级的 DAC-交联壳聚糖。通过氨基含量确定的交联度分别为 3.8%、8.3%和 12.1%。在 pH 2-9 范围内，DAC-交联壳聚糖表现出更高的稳定性，并且在长达 21 天的长期稳定性测试中没有发现细胞毒性。此外，DAC-交联壳聚糖在 pH 5.5 下表现出非常高的牛血清白蛋白 (BSA) 吸附能力，这是由于 DAC 与壳聚糖分子链之间的多点反应导致的氨基含量增加，尽管 DAC-交联壳聚糖的交联度较低。
