Yoshimoto H, Nagano N, Nishitoba T, Sato H, Miyata S, Kusaka M, Jing S B, Yamaguchi T
Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd., Gunma, Japan.
Nihon Yakurigaku Zasshi. 1995 Aug;106(2):113-22. doi: 10.1254/fpj.106.113.
The effects of chitosan-coated dialdehyde cellulose (chitosan DAC), a newly developed oral adsorbent of urea and ammonia, were examined in an in vitro adsorption study and in normal rats. Chitosan DAC showed high adsorption capacity for urea and ammonia in an in vitro study using the diluted supernatant of rat gastrointestinal fluid. In contrast, Kremezin, an oral charcoal adsorbent (AST-120), had little influence on these substances. In normal rats fed diets containing chitosan DAC (1, 2, 3, 4, 5, 7, and 10% content) for three weeks, increases in fecal wet weight, fecal dry weight and fecal water content were observed in a dose-dependent manner. In addition, chitosan DAC feeding increased fecal excretion of nitrogen and electrolytes (sodium, potassium and chloride ions) and decreased the apparent protein ratio in a dose-dependent manner. There were no obvious effects in serum parameters except that increased levels of protein and albumin and decreased levels of blood urea nitrogen, cholesterol and glucose were observed in rats fed a high concentration of chitosan DAC. In conclusion, these findings suggest the possibility that chitosan DAC treatment might be effective for improving chronic renal failure.
壳聚糖包被的二醛纤维素（壳聚糖DAC）是一种新开发的尿素和氨口服吸附剂，本研究在体外吸附实验和正常大鼠体内对其效果进行了检测。在使用大鼠胃肠液稀释上清液的体外研究中，壳聚糖DAC对尿素和氨显示出高吸附能力。相比之下，口服活性炭吸附剂克雷莫辛（AST - 120）对这些物质影响很小。在正常大鼠中，喂食含壳聚糖DAC（含量为1%、2%、3%、4%、5%、7%和10%）的饲料三周后，观察到粪便湿重、干重和含水量呈剂量依赖性增加。此外，喂食壳聚糖DAC使粪便中氮和电解质（钠离子、钾离子和氯离子）的排泄增加，并使表观蛋白比率呈剂量依赖性降低。除了喂食高浓度壳聚糖DAC的大鼠中观察到蛋白质和白蛋白水平升高、血尿素氮、胆固醇和葡萄糖水平降低外，血清参数没有明显影响。总之，这些发现提示壳聚糖DAC治疗可能对改善慢性肾衰竭有效。
