Liu Yao, Yang Guang, Ji Huanzhong, Xiang Tao, Luo En, Zhou Shaobing
State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China.
Key Laboratory of Advanced Technologies of Material, Minister of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
Colloids Surf B Biointerfaces. 2017 Jun 1;154:1-9. doi: 10.1016/j.colsurfb.2017.02.035. Epub 2017 Mar 1.
Mesenchymal stem cells (MSCs) are able to self-renew and differentiate into tissues of mesenchymal origin, making them to be significant for cell-based therapies, such as metabolic bone diseases and bone repair. Regulating the differentiation of MSCs is significant for bone regeneration. Electrospun fibers mimicking natural extracellular matrix (ECM), is an effective artificial ECM to regulate the behaviors and fates of MSCs. The aligned electrospun fibers can modulate polar cell pattern of bone mesenchymal stem cells, which leads to more obvious osteogenic differentiation. Apart from the topographic effect of electrospun fibers, mechanical cues can also intervene the cell behaviors. In this study, the osteogenic differentiation of rat bone mesenchymal stem cells was evaluated, which were cultured on aligned/random electrospun fiber mats materials under mechanical tension intervention. Scanning electron microscope and immune-fluorescent staining were used to directly observe the polarity changing of cellular morphology and cytoskeleton. The results proved that aligned electrospun fibers could be more conducive to promote osteogenic differentiation of rat bone mesenchymal stem cells and this promotion of osteogenic differentiation was enhanced by tension intervention. These results were correlated to the quantitative real-time PCR assay. In general, culturing rat bone mesenchymal stem cells on electrospun fibers under the intervention of mechanical tension is an effective way to mimic a more real cellular microenvironment.
间充质干细胞（MSCs）能够自我更新并分化为间充质来源的组织，这使得它们在基于细胞的疗法中具有重要意义，例如用于治疗代谢性骨疾病和骨修复。调节间充质干细胞的分化对骨再生具有重要意义。模仿天然细胞外基质（ECM）的电纺纤维是一种调节间充质干细胞行为和命运的有效人工细胞外基质。排列的电纺纤维可以调节骨间充质干细胞的极性细胞模式，从而导致更明显的成骨分化。除了电纺纤维的拓扑效应外，机械信号也可以干预细胞行为。在本研究中，评估了大鼠骨间充质干细胞在机械张力干预下在排列/随机电纺纤维垫材料上培养时的成骨分化情况。使用扫描电子显微镜和免疫荧光染色直接观察细胞形态和细胞骨架的极性变化。结果证明，排列的电纺纤维更有利于促进大鼠骨间充质干细胞的成骨分化，并且这种成骨分化的促进作用通过张力干预得到增强。这些结果与定量实时PCR分析相关。总体而言，在机械张力干预下在电纺纤维上培养大鼠骨间充质干细胞是模拟更真实细胞微环境的有效方法。
