Pinto Elsa, Anselmo Marisol, Calha Manuela, Bottrill Andrew, Duarte Isabel, Andrew Peter W, Faleiro Maria L
Faculdade de Ciências e Tecnologia, Centro de Investigação em Biomedicina, Universidade do Algarve Campus de Gambelas, 8005-139 Faro, Portugal.
Núcleo de Diabetologia, Nutrição e Doenças Metabólicas (NDNDM) do Hospital de Faro, 8000-386 Faro, Portugal.
Microbiology (Reading). 2017 Feb;163(2):161-174. doi: 10.1099/mic.0.000412.
In this study, the intestinal microbial proteome of children with established type 1 diabetes (T1D) was compared with the proteome of healthy children (Control) with the aim to identify differences in the activity of the intestinal microbiota that not only will contribute to a deeper knowledge of the functionality of the gut in these children but also may provide new approaches to improve the control of the disease. Faecal protein extracts collected from three T1D children (aged 9.3±0.6 years) and three Control children (aged 9.3±1.5 years) were analysed using a combination of 2D gel electrophoresis and spectral counting. The results evidenced markedly differences between the intestinal proteome of T1D children and the Control. The T1D microbial intestinal proteome was enriched with proteins of clostridial cluster XVa and cluster IV and Bacteroides. In contrast, the Control proteome was enriched with bifidobacterial proteins. In both groups, proteins with moonlight function were observed. Human proteins also distinguished the two groups with T1D children depleted in exocrine pancreatic enzymes.
在本研究中，将已确诊的1型糖尿病（T1D）患儿的肠道微生物蛋白质组与健康儿童（对照组）的蛋白质组进行比较，目的是确定肠道微生物群活性的差异，这不仅有助于更深入了解这些儿童肠道的功能，还可能提供改善疾病控制的新方法。使用二维凝胶电泳和光谱计数相结合的方法，对从三名T1D患儿（年龄9.3±0.6岁）和三名对照儿童（年龄9.3±1.5岁）收集的粪便蛋白质提取物进行分析。结果表明，T1D患儿与对照组的肠道蛋白质组存在明显差异。T1D微生物肠道蛋白质组富含梭菌属XVa簇、IV簇和拟杆菌属的蛋白质。相比之下，对照组蛋白质组富含双歧杆菌属蛋白质。在两组中均观察到具有兼职功能的蛋白质。人类蛋白质也区分了两组，T1D患儿的外分泌胰腺酶含量较低。
