Xie Yijing, Harsan Laura-Adela, Bienert Thomas, Kirch Robert D, von Elverfeldt Dominik, Hofmann Ulrich G
Section of Neuroelectronic Systems, Department of General Neurosurgery, Medical Center University of Freiburg, Engesserstraβe 4, 79108 Freiburg, Germany; Translational Imaging Group, Centre for Medical Image Computing, Department of Medical Physics and Biomedical Engineering, University College London, WC1E 6BT London, UK.
Department of Diagnostic Radiology, Medical Center University of Freiburg, Hugstetter Straβe 55, 79106 Freiburg, Germany; Laboratory of Engineering, Informatics and Imaging (ICube), Integrative Multimodal Imaging in Healthcare (IMIS), UMR 7357, University of Strasbourg, 4 Rue Kirschleger, 67000 Strasbourg, France; Department of Biophysics and Nuclear Medicine, Hautepierre Hospital, University Hospitals of Strasbourg, 1, Avenue Molière, 67098 Strasbourg Cedex, France.
Biomed Opt Express. 2017 Jan 5;8(2):593-607. doi: 10.1364/BOE.8.000593. eCollection 2017 Feb 1.
OCT has been demonstrated as an efficient imaging modality in various biomedical and clinical applications. However, there is a missing link with respect to the source of contrast between OCT and other modern imaging modalities, no quantitative comparison has been demonstrated between them, yet. We evaluated, to our knowledge, for the first time OCT measurement of rat brain with our previously proposed forward imaging method by both qualitatively and quantitatively correlating OCT with the corresponding T1-weighted and T2-weighted magnetic resonance images, fiber density map (FDM), and two types of histology staining (cresyl violet and acetylcholinesterase AchE), respectively. Brain anatomical structures were identified and compared across OCT, MRI and histology imaging modalities. Noticeable resemblances corresponding to certain anatomical structures were found between OCT and other image profiles. Correlation was quantitatively assessed by estimating correlation coefficient (R) and mutual information (MI). Results show that the 1-D OCT measurements in regards to the intensity profile and estimated attenuation factor, do not have profound linear correlation with the other image modalities suggested from correlation coefficient estimation. However, findings in mutual information analysis demonstrate that there are markedly high MI values in OCT-MRI signals.
光学相干断层扫描（OCT）已被证明是一种在各种生物医学和临床应用中有效的成像方式。然而，关于OCT与其他现代成像方式之间的对比度来源，还存在一个缺失环节，目前尚未对它们进行定量比较。据我们所知，我们首次使用先前提出的前向成像方法，通过将OCT与相应的T1加权和T2加权磁共振图像、纤维密度图（FDM）以及两种组织学染色（甲酚紫和乙酰胆碱酯酶AchE）进行定性和定量关联，对大鼠大脑进行了OCT测量。在OCT、MRI和组织学成像方式之间识别并比较了脑解剖结构。在OCT和其他图像轮廓之间发现了与某些解剖结构相对应的明显相似之处。通过估计相关系数（R）和互信息（MI）对相关性进行了定量评估。结果表明，就强度分布和估计的衰减因子而言，一维OCT测量与相关系数估计所表明的其他图像方式没有显著的线性相关性。然而，互信息分析的结果表明，OCT-MRI信号中存在明显较高的MI值。
